Cham Kai Lin, Soga Tomoko, Parhar Ishwar S
Brain Research Institute, School of Medicine and Health Sciences, Monash University Malaysia, Bandar Sunway, Malaysia.
Front Neuroanat. 2018 Dec 6;12:109. doi: 10.3389/fnana.2018.00109. eCollection 2018.
Serotonin (5-hydroxytryptamine, 5-HT) is one of the major neurotransmitters, modulating diverse behaviours and physiological functions. Really interesting new gene (RING) finger protein 38 (RNF38) is an E3 ubiquitin ligase whose function remains unclear. A recent study has shown a possible regulatory relationship between RNF38 and the 5-HT system. Therefore, to gain insight into the role of RNF38 in the central 5-HT system, we identified the neuroanatomical location of 5-HT positive cells and investigated the relationship between RNF38 and the 5-HT system in the brain of the Nile tilapia, . Immunocytochemistry revealed three neuronal populations of 5-HT in the brain of tilapia; the paraventricular organ (PVO), the dorsal and ventral periventricular pretectal nuclei (PPd and PPv), and, the superior and inferior raphe (SR and IR). The 5-HT neuronal number was highest in the raphe (90.4 in SR, 284.6 in IR), followed by the pretectal area (22.3 in PPd, 209.8 in PPv). Double-label immunocytochemistry showed that the majority of 5-HT neurons express RNF38 nuclear proteins (66.5% in PPd; 77.9% in PPv; 35.7% in SR; 49.1% in IR). These findings suggest that RNF38 could be involved in E3 ubiquitination in the central 5-HT system.
血清素（5-羟色胺，5-HT）是主要的神经递质之一，可调节多种行为和生理功能。真有趣新基因（RING）指蛋白38（RNF38）是一种E3泛素连接酶，其功能尚不清楚。最近的一项研究表明RNF38与5-HT系统之间可能存在调节关系。因此，为了深入了解RNF38在中枢5-HT系统中的作用，我们确定了5-HT阳性细胞的神经解剖位置，并研究了尼罗罗非鱼脑中RNF38与5-HT系统之间的关系。免疫细胞化学显示罗非鱼脑中5-HT有三个神经元群；室旁器官（PVO）、背侧和腹侧室周顶盖前核（PPd和PPv）以及上、下中缝核（SR和IR）。5-HT神经元数量在中缝核中最高（SR中为90.4，IR中为284.6），其次是顶盖前区（PPd中为22.3，PPv中为209.8）。双标记免疫细胞化学显示，大多数5-HT神经元表达RNF38核蛋白（PPd中为66.5%；PPv中为77.9%；SR中为35.7%；IR中为49.1%）。这些发现表明RNF38可能参与中枢5-HT系统中的E3泛素化作用。
