阿片类药物使用与血液 DNA 甲基化：全基因组关联荟萃分析。
Opioid medication use and blood DNA methylation: epigenome-wide association meta-analysis.
机构信息
Epidemiology Branch, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.
Department of Health and Human Services, Framingham Heart Study, National Heart, Lung, and Blood Institute, National Institutes of Health, Framingham, MA 01702, USA.
出版信息
Epigenomics. 2022 Dec;14(23):1479-1492. doi: 10.2217/epi-2022-0353. Epub 2023 Jan 26.
To identify differential methylation related to prescribed opioid use. This study examined whether blood DNA methylation, measured using Illumina arrays, differs by recent opioid medication use in four population-based cohorts. We meta-analyzed results (282 users; 10,560 nonusers) using inverse-variance weighting. Differential methylation (false discovery rate <0.05) was observed at six CpGs annotated to the following genes: , , , , and . Integrative epigenomic analyses linked implicated loci to regulatory elements in blood and/or brain. Additionally, 74 CpGs were differentially methylated in males or females. Methylation at significant CpGs correlated with gene expression in blood and/or brain. This study identified DNA methylation related to opioid medication use in general populations. The results could inform the development of blood methylation biomarkers of opioid use.
为了确定与处方类阿片类药物使用相关的差异甲基化。本研究通过四个基于人群的队列，使用 Illumina 芯片检测血液 DNA 甲基化水平，来评估近期是否有阿片类药物的使用。我们采用逆方差加权法对结果（282 名使用者；10560 名非使用者）进行了荟萃分析。在六个 CpG 位点观察到差异甲基化（错误发现率 <0.05），这些 CpG 位点注释到以下基因：、、、、和。综合表观基因组分析将涉及的基因座与血液和/或大脑中的调控元件联系起来。此外，在男性或女性中，74 个 CpG 存在差异甲基化。在有统计学意义的 CpG 位点的甲基化与血液和/或大脑中的基因表达相关。本研究在一般人群中确定了与阿片类药物使用相关的 DNA 甲基化。这些结果可能为开发阿片类药物使用的血液甲基化生物标志物提供信息。
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