Vanwyk Anna M, Kotzé Antoinette, Paul Grobler J, Van Vuuren Bettine Janse, Barrow Lisa N, Dalton Desiré L
National Zoological Gardens, South African Biodiversity Institute, P.O. Box 754, Pretoria 0001, South Africa.
J Genet. 2018 Nov 1;97(5):e101-e109.
The bluewildebeest () is distributed throughout southern and east Africa while the black wildebeest () is endemic to South Africa and was driven to near extinction in the early 1900s due to hunting pressure and disease outbreaks. Extensive translocation of both species throughout South Africa is threatening the genetic integrity of blue and blackwilde beest. To effectively manage these species, genetic tools that can be used to detect hybrid individuals, identify genetically unique subpopulations and determine the levels of genetic diversity are required. In this study, 11 microsatellite markers were developed for wildebeest through next-generation sequencing. The microsatellite loci displayed 2.00-4.14 alleles, unbiased heterozygosity values ranged from 0.32 to 0.60 and observed heterozygosity values ranged from 0.26 to 0.52. The comparatively high level of polymorphism observed in the microsatellite markers indicates that these markers can contribute significantly to our knowledge of population genetic structure, relatedness, genetic diversity and hybridization in these species.
蓝角马（）分布于非洲南部和东部，而黑角马（）是南非特有的物种，在20世纪初由于狩猎压力和疾病爆发而濒临灭绝。这两个物种在南非各地的广泛迁移正威胁着蓝角马和黑角马的遗传完整性。为了有效管理这些物种，需要能够用于检测杂交个体、识别遗传上独特的亚种群并确定遗传多样性水平的遗传工具。在本研究中，通过下一代测序为角马开发了11个微卫星标记。微卫星位点显示出2.00 - 4.14个等位基因，无偏杂合度值范围为0.32至0.60，观察到的杂合度值范围为0.26至0.52。在微卫星标记中观察到的相对较高水平的多态性表明，这些标记可以极大地增进我们对这些物种的种群遗传结构、亲缘关系、遗传多样性和杂交情况的了解。
