Laboratorio de Fitoquímica, UBIPRO, FES-Iztacala, Unidad Nacional Autónoma de México, Av. de los Barrios No.1, Los Reyes Iztacala, Tlalnepantla, 54090, Estado de México, México.
Laboratorio de Histología, UMF, FES-Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios No.1, Los Reyes Iztacala, Tlalnepantla, 54090, Edo. de México, México.
Food Chem Toxicol. 2019 Feb;124:411-422. doi: 10.1016/j.fct.2018.12.024. Epub 2018 Dec 18.
Dyssodia tagetiflora is known as 'Tzaracata' and 'flor de muerto'. Recently, D. tagetiflora has been reported to have antioxidant activities in its polar extracts as well as insecticidal activities. Hyperoside (1), avicularin (2) and avicularin acetate (3) have been isolated previously. However, the temporary variation in glycoside flavonoids biosynthesis, as well as antibacterial and chemoprotective activities, have not been reported. The amount of 1, 2 and 3 in the different collections was characterized by HPLC-MS. Two new C-glycosides were characterized, quercetin-4'-methyl ether 6-C glucoside (A1) and quercetin-4'-methyl ether 8-C glucoside (A2), as well as [2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4-oxochromen-3-yl]3,4,5-trihydroxyoxane-2,6-dicarboxylate (A3). This is the first report of the presence of C-C flavonoid glycosides compounds in the genus Dyssodia. Hyperoside was the majority compound at all collections. The methanolic extracts of August 2016 and October 2017 were active against Micrococcus luteus and Bacillus subtillis. The methanolic extract has chemoprotective effects because, when applied topically in SKH-1 mice, it decreases the severity of epidermal damage induced by acute exposure to ultraviolet radiation. In addition, cutaneous photocarcinogenesis was decreased in mice treated with the extract. The methanolic extract of D. tagetiflora has chemoprotective properties by decreasing the damage caused by acute and chronic exposure to UV in mice.
多穗金腰花被称为“Tzaracata”和“死花”。最近，据报道，其极性提取物具有抗氧化活性，并且具有杀虫活性。先前已经分离出 hyperoside (1)、avicularin (2) 和 avicularin acetate (3)。然而，糖苷类黄酮生物合成的暂时变化以及抗菌和化学保护活性尚未报道。不同采集物中 1、2 和 3 的含量通过 HPLC-MS 进行了表征。鉴定出两种新的 C-糖苷，槲皮素-4'-甲醚 6-C 葡萄糖苷 (A1) 和槲皮素-4'-甲醚 8-C 葡萄糖苷 (A2)，以及[2-(3,4-二羟基苯基)-5,7-二羟基-4-氧代色原酮-3-基]3,4,5-三羟基氧杂环己烷-2,6-二甲酸酯 (A3)。这是首次在 Dyssodia 属中发现 C-C 类黄酮糖苷化合物。在所有采集物中，hyperoside 都是主要化合物。2016 年 8 月和 2017 年 10 月的甲醇提取物对微球菌和枯草芽孢杆菌具有活性。甲醇提取物具有化学保护作用，因为当局部应用于 SKH-1 小鼠时，它可降低急性暴露于紫外线辐射引起的表皮损伤的严重程度。此外，用提取物处理的小鼠皮肤光致癌作用降低。多穗金腰花甲醇提取物通过减少急性和慢性暴露于 UV 对小鼠造成的损伤而具有化学保护特性。
