Ding Y, Shao J, Hua K, Wang X
Department of Gynecology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.
Department of Urology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Transplant Proc. 2018 Dec;50(10):3881-3886. doi: 10.1016/j.transproceed.2018.06.038. Epub 2018 Jun 30.
A rat model of right whole ovary transplantation is difficult to establish because of an unfit location or absence of the right ovarian artery. The purpose of this study is to develop a rat model of left whole ovary transplantation.
Two methods of whole ovary transplantation were performed. In Group A (n = 40), the rats underwent right whole ovary grafting via conventional orthotopic allotransplant, with donor abdominal aorta and inferior vena cava end-to-side anastomoses to the recipient abdominal aorta and inferior vena cava. In Group B (n = 40), the rats underwent left whole ovary graft via orthotopic allotransplant, with donor abdominal aorta and left renal vein end-to-side anastomoses to the recipient abdominal aorta and inferior vena cava. Bilateral native ovariectomy in the recipient was performed immediately in both groups. The operative times, recipient survival rates, and transplanted ovarian function were measured.
There was no significant difference in the success rates of transplant between the 2 groups, with 92.5% and 90% success, respectively. All the living recipients recovered ovarian function. However, the operative time for the donor, the vein anastomosis time, and the cold ischemia time decreased significantly in Group B (P < .05).
The modified method of left whole ovary transplantation is reliable and easy to perform, which could significantly shorten the times of graft harvest and cold ischemia. The method is a good supplement and improvement of the conventional model of rat whole ovary transplantation.
由于右侧卵巢动脉位置不合适或不存在，建立大鼠右侧全卵巢移植模型较为困难。本研究的目的是建立大鼠左侧全卵巢移植模型。
采用两种全卵巢移植方法。A组（n = 40）大鼠通过传统原位同种异体移植进行右侧全卵巢移植，供体腹主动脉和下腔静脉与受体腹主动脉和下腔静脉行端侧吻合。B组（n = 40）大鼠通过原位同种异体移植进行左侧全卵巢移植，供体腹主动脉和左肾静脉与受体腹主动脉和下腔静脉行端侧吻合。两组受体均立即行双侧原位卵巢切除术。测量手术时间、受体存活率和移植卵巢功能。
两组移植成功率无显著差异，分别为92.5%和90%。所有存活的受体均恢复了卵巢功能。然而，B组供体手术时间、静脉吻合时间和冷缺血时间均显著缩短（P < .05）。
改良的左侧全卵巢移植方法可靠且易于操作，可显著缩短移植物获取时间和冷缺血时间。该方法是大鼠全卵巢移植传统模型的良好补充和改进。
