Hao Lin, Jia Qiucheng, Tian Nana, Tang Huimin, Liu Gaoyuan, Li Yachai, Geng Min, Chen Wanying, Chen Jiming, Cai Zhihui
Department of Gynaecology, Affiliated Hospital of Hebei University, Baoding, 071000, Hebei, China.
Department of Gynecology, The Affiliated Changzhou Second People'S Hospital of Nanjing Medical University, Changzhou, 213000, Jiangsu, China.
Sci Rep. 2025 Mar 19;15(1):9500. doi: 10.1038/s41598-025-94456-6.
In this study, ovarian transplantation at different sites was performed on the rat model of premature ovarian failure, to explore the effect on the recovery of ovarian function, and to evaluate the application value in the treatment of ovarian function decline caused by the application of chemotherapy drugs. Thirty 2-month-old female SD rats of SPF grade were randomly divided into five groups, and both ovaries in the model group were treated with cisplatin (11 mg/kg) for modelling. After successful modelling, another 18 rats were taken as donor rats: group A was the control group, group B was the model group and group CDE was the experimental group. Ovary transplantation was performed using muscle, subcutaneous and renal peritoneum, respectively, and the rats were monitored for their general condition, estrous cycle and serum hormone levels after surgery. At the end of the observation period, the rats were executed and follicular development was observed.We confirm that all methods are carried out in accordance with the relevant guidelines and regulations.After 14 days of xenotransplantation, the general condition of the rats in the experimental group was better than that of the model group. Serum E2 and AMH levels were significantly higher than those in the model group (P = 0.000), and FSH levels were significantly lower than those in the control group (P = 0.000). Cisplatin can be used to successfully establish the rat POF model; the three transplantation sites (muscle, subcutaneous and renal peritoneum) selected in this experiment can make the transplanted ovarian tissue survive and successfully restore the endocrine function of the body, and the effect of which showed no significant difference.
本研究对卵巢早衰大鼠模型进行不同部位的卵巢移植，以探讨其对卵巢功能恢复的影响，并评估其在治疗化疗药物所致卵巢功能减退中的应用价值。将30只2月龄SPF级雌性SD大鼠随机分为五组，模型组双侧卵巢用顺铂（11mg/kg）处理进行造模。造模成功后，选取另外18只大鼠作为供体大鼠：A组为对照组，B组为模型组，CDE组为实验组。分别采用肌肉、皮下和肾周腹膜进行卵巢移植，术后监测大鼠的一般状况、发情周期和血清激素水平。观察期结束时，处死大鼠并观察卵泡发育情况。我们确认所有方法均按照相关指南和规定进行。异种移植14天后，实验组大鼠的一般状况优于模型组。血清E2和AMH水平显著高于模型组（P = 0.000），FSH水平显著低于对照组（P = 0.000）。顺铂可成功建立大鼠POF模型；本实验选取的三个移植部位（肌肉、皮下和肾周腹膜）均可使移植的卵巢组织存活并成功恢复机体的内分泌功能，且三者效果无显著差异。
