Lebedev V S, Rezepov F F, Chebotareva S V, Bondareva V Ia, Selezneva V P
Zh Mikrobiol Epidemiol Immunobiol. 1988 Aug(8):83-7.
On the basis of the experimentally established dependence of the degree of binding of diphtheria toxoid with standard diphtheria antitoxin on the duration of their joint incubation with the maximum binding occurring in 3-4 hours of incubation at 37 degrees C, the method of the in vitro determination of the antigenic activity of diphtheria toxoid in liquid and adsorbed preparations is proposed. The method is based on the principle of double binding with the use of diphtheria antigenic (toxoid) erythrocyte diagnosticum. The antigenic activity of diphtheria toxoid, evaluated by the degree of its maximum binding with diphtheria antitoxin, correlated with its antitoxin-binding activity in animal experiments and did not correlate with its flocculating activity. The antigenic activity of diphtheria toxoid in adsorbed preparations, evaluated by their maximum binding with standard diphtheria antitoxin, was shown to be closely related to the immunogenic potency of these vaccines in animals.
根据实验确定的白喉类毒素与标准白喉抗毒素结合程度对其在37℃联合孵育持续时间的依赖性，其中最大结合发生在孵育3 - 4小时，提出了体外测定液体和吸附制剂中白喉类毒素抗原活性的方法。该方法基于使用白喉抗原（类毒素）红细胞诊断制剂进行双重结合的原理。通过白喉类毒素与白喉抗毒素最大结合程度评估的白喉类毒素抗原活性，在动物实验中与其抗毒素结合活性相关，而与其絮凝活性无关。通过吸附制剂与标准白喉抗毒素的最大结合评估的白喉类毒素抗原活性，被证明与这些疫苗在动物中的免疫原性密切相关。
