Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan.
Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan; Department of Life Sciences, National Central University, Taoyuan, Taiwan.
Cytokine. 2019 Feb;114:11-17. doi: 10.1016/j.cyto.2018.11.026. Epub 2018 Dec 20.
Suppressors of cytokine signaling (SOCS), particularly SOCS-3, allow discrimination of patients with active tuberculosis (TB) from healthy subjects in a gender- and age-dependent manner. However, no information is available on whether single nucleotide polymorphisms (SNPs) in the SOCS-3 gene occur in patients with TB. This study was designed to investigate SOCS-3 SNPs in association with susceptibility to TB in the Taiwanese population.
Four SNPs in the SOCS-3 gene located at rs8064821, rs4969168, rs2280148, and rs35037722 were studied by the TaqMan SNP Genotyping assay in 200 healthy and 210 TB patients enrolled in 2015-2018.
Significant differences were not detected in genotype frequencies or odds ratios (ORs) between healthy and TB patients for any of the four polymorphisms. The lack of significant differences was also found when the patients were stratified by sex. However, males exhibited GG homozygous at rs35037722 in association with susceptibility to TB after the OR analysis was adjusted for age. For rs8064821, AA and AC genotypes were associated with TB susceptibility in patients ≤ 65 years old compared to CC genotype, whereas older subjects had no such association.
The results suggest that particular SOCS-3 SNPs are dependent on gender or age to influence TB susceptibility in the Han Taiwanese.
细胞因子信号转导抑制因子（SOCS），尤其是 SOCS-3，能够以性别和年龄依赖的方式区分活动性肺结核（TB）患者和健康受试者。然而，目前尚不清楚 SOCS-3 基因中的单核苷酸多态性（SNPs）是否存在于 TB 患者中。本研究旨在探讨 SOCS-3 SNPs 与台湾人群中 TB 易感性的关系。
在 2015-2018 年间，使用 TaqMan SNP 基因分型检测方法，对位于 rs8064821、rs4969168、rs2280148 和 rs35037722 的 SOCS-3 基因中的 4 个 SNPs 进行研究，共纳入 200 名健康对照者和 210 名 TB 患者。
在任何一种多态性中，健康对照者和 TB 患者之间的基因型频率或比值比（OR）均无显著差异。当按性别对患者进行分层时，也未发现显著差异。然而，在调整年龄因素后，男性在 rs35037722 中表现为 GG 纯合子与 TB 易感性相关。对于 rs8064821，AA 和 AC 基因型与≤65 岁患者的 TB 易感性相关，而与 CC 基因型相比，年龄较大的患者则没有这种相关性。
结果表明，特定的 SOCS-3 SNPs 依赖于性别或年龄来影响汉族台湾人群的 TB 易感性。
