Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China; State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, 510070, China; Guangdong Bide Biotech CO. L.T.D., Guangzhou, Guangdong, China.
Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China.
Biochem Biophys Res Commun. 2019 Jan 29;509(1):125-132. doi: 10.1016/j.bbrc.2018.12.082. Epub 2018 Dec 21.
Bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) gene encodes a transmembrane protein and is involved in multiple physiological and pathological processes, such as inflammatory response, tumor development and progression, cell proliferation and differentiation. A previous study suggested that BAMBI may interact with the Wnt/β-catenin signaling pathway via promoting β-catenin nuclear translocation associated with C2C12 myogenic myoblast differentiation. However, its biological function in skeletal muscle still remains unknown and requires further characterization. The present work sought to investigate its biological function in skeletal muscle, especially the physiological roles of BAMBI during skeletal muscle growth and regeneration. Our current work suggests that BAMBI protein is highly expressed in skeletal muscle and is only detected in cytosolic fraction in the resting muscle. Moreover, BAMBI protein is co-localized in fast-twitch (glycolytic) fibers, but not in slow-twitch (oxidative) fibers. Comparing with the cytosolic trapping in resting muscle, BAMBI protein is enriched on cellular membrane during the muscle growth and regeneration, suggesting that BAMBI-mediated a significant signaling pathway may be an essential part of muscle growth and regeneration.
骨形态发生蛋白和激活素膜结合抑制剂（BAMBI）基因编码一种跨膜蛋白，参与多种生理和病理过程，如炎症反应、肿瘤发生和进展、细胞增殖和分化。先前的研究表明，BAMBI 可能通过促进与 C2C12 成肌细胞分化相关的β-连环蛋白核转位与 Wnt/β-连环蛋白信号通路相互作用。然而，其在骨骼肌中的生物学功能尚不清楚，需要进一步表征。本工作旨在研究其在骨骼肌中的生物学功能，特别是 BAMBI 在骨骼肌生长和再生过程中的生理作用。我们目前的工作表明，BAMBI 蛋白在骨骼肌中高度表达，仅在静息肌肉的胞质部分检测到。此外，BAMBI 蛋白在快肌（糖酵解）纤维中共定位，但不在慢肌（氧化）纤维中。与静息肌肉中的胞质捕获相比，BAMBI 蛋白在肌肉生长和再生过程中富集在细胞膜上，这表明 BAMBI 介导的信号通路可能是肌肉生长和再生的重要组成部分。
