Analytical Division, Department of Chemistry, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
Analytical Division, Department of Chemistry, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
Biosens Bioelectron. 2019 Feb 15;127:10-18. doi: 10.1016/j.bios.2018.11.055. Epub 2018 Dec 7.
A novel molecularly imprinted polymer-capped acrylated nitrogen doped graphene quantum dots and bimetallic Au/Ag core-shell was synthesized to serve as a sensing nano-hybrid film for the detection of an anticancerous drug, hydroxyurea. This exploited the use of a functionalized nitrogen doped graphene quantum dots iniferter. This initiated the polymerization, following "surface grafting-from" approach, over the surface of a screen-printed carbon electrode to obtain requisite stability and selectivity of the measurement. Herein, nitrogen doped graphene quantum dots were prepared utilizing the degree of dehydration/carbonization of citric acid (carbon skeleton) and urea (nitrogen dopant) as source materials. This provided an efficient sensor platform anchoring bimetallic Au/Ag core-shell on its surface. The nano-assembly of acrylated nitrogen doped graphene quantum dots and bimetallic Au/Ag core-shell@imprinted polymer actually amplified the electrode kinetics by improving the diffusion coefficient (20-fold) and electron-transfer kinetics (5-fold), in comparison to the simple bimetallic Au/Ag core-shell decorated imprinted sensor. Under optimized conditions of differential pulse anodic stripping voltammetric transduction, a linear relationship between the current and the concentration was obtained in the range of 0.62-102.33 ng mL for hydroxyurea. The detection limit was observed to be 0.07 ng mL in blood plasma, without having any matrix effect, cross-reactivity, and false-positives. The proposed sensor assures its clinical applicability for the treatment of cancer.
一种新型的分子印迹聚合物- capped 丙烯酰化氮掺杂石墨烯量子点和双金属 Au/Ag 核壳被合成，作为一种用于检测抗癌药物羟基脲的传感纳米杂化膜。这利用了功能化氮掺杂石墨烯量子点引发剂的作用。采用“表面接枝-从”方法，在丝网印刷碳电极表面引发聚合，以获得所需的测量稳定性和选择性。在此，氮掺杂石墨烯量子点是利用柠檬酸（碳骨架）和尿素（氮掺杂剂）的脱水/碳化程度作为原料制备的。这为表面锚定双金属 Au/Ag 核壳提供了一个有效的传感器平台。与简单的双金属 Au/Ag 核壳修饰印迹传感器相比，丙烯酰化氮掺杂石墨烯量子点和双金属 Au/Ag 核壳@印迹聚合物的纳米组装实际上通过提高扩散系数（20 倍）和电子转移动力学（5 倍）来放大电极动力学。在差分脉冲阳极溶出伏安检测的优化条件下，电流与浓度之间在 0.62-102.33ng mL 范围内呈现线性关系。在血浆中，检测限观察到为 0.07ng mL，没有任何基质效应、交叉反应和假阳性。该传感器确保了其在癌症治疗中的临床适用性。
