产前家庭空气污染改变脐血单个核细胞线粒体 DNA 拷贝数：性别特异性关联。
Prenatal Household Air Pollution Alters Cord Blood Mononuclear Cell Mitochondrial DNA Copy Number: Sex-Specific Associations.
机构信息
Kintampo Health Research Centre, Ghana Health Service, Brong Ahafo Region, P. O. Box 200, Kintampo Ghana.
Department of Environmental Health Sciences, Columbia University Mailman School of Public Health, New York, NY 10032, USA.
出版信息
Int J Environ Res Public Health. 2018 Dec 22;16(1):26. doi: 10.3390/ijerph16010026.
BACKGROUND
Associations between prenatal household air pollution (HAP) exposure or cookstove intervention to reduce HAP and cord blood mononuclear cell (CBMC) mitochondrial deoxyribonucleic acid copy number (mtDNAcn), an oxidative stress biomarker, are unknown.
MATERIALS AND METHODS
Pregnant women were recruited and randomized to one of two cookstove interventions, including a clean-burning liquefied petroleum gas (LPG) stove, or control. Prenatal HAP exposure was determined by serial, personal carbon monoxide (CO) measurements. CBMC mtDNAcn was measured by quantitative polymerase chain reaction. Multivariable linear regression determined associations between prenatal CO and cookstove arm on mtDNAcn. Associations between mtDNAcn and birth outcomes and effect modification by infant sex were explored.
RESULTS
LPG users had the lowest CO exposures ( = 0.02 by ANOVA). In boys only, average prenatal CO was inversely associated with mtDNAcn (β = -14.84, SE = 6.41, = 0.03, per 1ppm increase in CO). When examined by study arm, LPG cookstove had the opposite effect in all children (LPG β = 19.34, SE = 9.72, = 0.049), but especially boys (β = 30.65, SE = 14.46, = 0.04), as compared to Control. Increased mtDNAcn was associated with improved birth outcomes.
CONCLUSIONS
Increased prenatal HAP exposure reduces CBMC mtDNAcn, suggesting cumulative prenatal oxidative stress injury. An LPG stove intervention may reverse this effect. Boys appear most susceptible.
背景
产前家庭空气污染（HAP）暴露或减少 HAP 的炊具干预与脐带血单个核细胞（CBMC）线粒体脱氧核糖核酸（mtDNAcn）拷贝数（一种氧化应激生物标志物）之间的关联尚不清楚。
材料和方法
招募孕妇并将其随机分为两种炊具干预组之一，包括燃烧清洁的液化石油气（LPG）炉或对照组。通过连续的个人一氧化碳（CO）测量来确定产前 HAP 暴露。通过定量聚合酶链反应测量 CBMC mtDNAcn。多变量线性回归确定产前 CO 与 mtDNAcn 之间的关联与炊具臂。探讨了 mtDNAcn 与出生结局之间的关系及其与婴儿性别之间的相互作用。
结果
LPG 用户的 CO 暴露最低（方差分析 = 0.02）。仅在男孩中，平均产前 CO 与 mtDNAcn 呈负相关（β = -14.84，SE = 6.41， = 0.03，每增加 1ppm CO）。当按研究臂检查时，LPG 炊具在所有儿童中都有相反的作用（LPGβ= 19.34，SE = 9.72， = 0.049），但尤其是男孩（β= 30.65，SE = 14.46， = 0.04），与对照相比。增加的 mtDNAcn 与改善的出生结局相关。
结论
产前 HAP 暴露增加会降低 CBMC mtDNAcn，表明累积的产前氧化应激损伤。LPG 炉干预可能会逆转这种效果。男孩似乎最容易受到影响。
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