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Symbiosis Technologies for Insect Control (SymbioTIC). Plateforme de Recherche Cyroi, 2 rue Maxime Rivière, 97490 Ste Clotilde, Ste Clotilde, France.
Parasit Vectors. 2018 Dec 24;11(Suppl 2):658. doi: 10.1186/s13071-018-3212-y.
Aedes albopictus is an invasive mosquito species of global medical concern as its distribution has recently expanded to Africa, the Americas and Europe. In the absence of prophylaxis protecting human populations from emerging arboviruses transmitted by this mosquito species, the most straightforward control measures rely on the suppression or manipulation of vector natural populations. A number of environmental-friendly methods using mass releases of sterilizing males are currently under development. However, these strategies are still lacking an efficient sexing method required for production of males at an industrial scale.
We present the first Genetic Sexing Strain (GSS) in Ae. albopictus, hereafter referred as Tikok, obtained by sex linkage of the rdl gene conferring dieldrin resistance. Hatch rate, larval survival and sex ratio were followed during twelve generations. The use of dieldrin at the third larval stage allowed selecting 98 % of males on average.
A good production rate of Tikok males makes this GSS suitable for any control method based on mass production of Ae. albopictus males. Despite limitations resulting from reduced egg hatch as well as the nature of the used insecticide, the construction of this GSS paves the way for industrial sex separation of Ae. albopictus.
白纹伊蚊是一种具有全球医学意义的入侵性蚊子物种，其分布范围最近已扩展到非洲、美洲和欧洲。由于缺乏预防措施来保护人类免受这种蚊子传播的新兴虫媒病毒的侵害，最直接的控制措施依赖于抑制或操纵病媒自然种群。目前正在开发许多使用大量释放绝育雄蚊的环保方法。然而，这些策略仍然缺乏生产工业规模雄蚊所需的有效性别鉴定方法。
我们提出了白纹伊蚊的第一个遗传性别鉴定品系（GSS），以下称为 Tikok，它通过赋予二氯苯醚菊酯抗性的 rdl 基因的性连锁获得。在十二个世代中，我们跟踪了孵化率、幼虫存活率和性别比例。在第三龄幼虫阶段使用二氯苯醚菊酯平均可选择 98%的雄性。
Tikok 雄蚊的高产量使得该 GSS 适合任何基于大量生产白纹伊蚊雄蚊的控制方法。尽管由于卵孵化减少以及所用杀虫剂的性质而存在限制，但该 GSS 的构建为白纹伊蚊的工业性别分离铺平了道路。
