利用傅里叶变换红外光谱法深入研究氯霉素和罗红霉素对藻类的毒性作用。
New insight into the toxic effects of chloramphenicol and roxithromycin to algae using FTIR spectroscopy.
机构信息
State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; The Environmental Research Institute, MOE Key Laboratory of Environmental Theoretical Chemistry, South China Normal University, Guangzhou 510006, China; University of Chinese Academy of Sciences, Beijing 100049, China.
The Environmental Research Institute, MOE Key Laboratory of Environmental Theoretical Chemistry, South China Normal University, Guangzhou 510006, China.
出版信息
Aquat Toxicol. 2019 Feb;207:197-207. doi: 10.1016/j.aquatox.2018.12.017. Epub 2018 Dec 21.
Antibiotics have been frequently detected in the aquatic environment, and they may affect aquatic organisms such as algae. Here we investigated toxicity of chloramphenicol (CAP) and roxithromycin (ROX) on four species of green algae (Pseudokirchneriella subcapitata, Scenedesmus quadricauda, Scenedesmus obliquus, and Scenedesmus acuminatus) at biochemical level by Fourier transform infrared spectroscopy (FTIR). The results revealed that both CAP and ROX had negative effects on algal growth and caused alterations of biochemical components. The toxic effects varied among the four algal species and S. acuminatus was found to be less sensitive than the other three species to the antibiotics. Even with similar mechanism of action, ROX displayed more adverse effects to algae than CAP. Both antibiotics could affect algae by inhibiting fatty acid synthesis and promoting protein and DNA aggregation, thus leading to accumulation of lipid peroxidation products, increment of the loose β-sheet structure protein and transformation of B-DNA to Z-DNA. The findings from this study revealed the toxic mechanism of antibiotics to algae at the biochemical level.
抗生素经常在水环境中被检测到，它们可能会对藻类等水生生物产生影响。在这里，我们通过傅里叶变换红外光谱（FTIR）技术研究了氯霉素（CAP）和罗红霉素（ROX）对四种绿藻（Pseudokirchneriella subcapitata、Scenedesmus quadricauda、Scenedesmus obliquus 和 Scenedesmus acuminatus）的生化毒性。结果表明，CAP 和 ROX 都对藻类生长有负面影响，并导致生化成分的改变。四种藻类的毒性效应不同，并且发现 S. acuminatus 对抗生素的敏感性低于其他三种藻类。即使作用机制相似，ROX 对藻类的不良影响也大于 CAP。这两种抗生素都可以通过抑制脂肪酸合成和促进蛋白质和 DNA 聚集来影响藻类，从而导致脂质过氧化产物的积累、松散β-折叠结构蛋白的增加和 B-DNA 向 Z-DNA 的转变。本研究的结果揭示了抗生素对藻类的生化毒性机制。
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