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用于小鼠肾脏层粘连蛋白包埋后免疫荧光染色的LR-White和LR-金树脂。

LR-White and LR-Gold resins for postembedding immunofluorescence staining of laminin in mouse kidney.

作者信息

Herken R, Fussek M, Barth S, Götz W

机构信息

Abteilung Histologie, Georg-August Universität Göttingen, Germany.

出版信息

Histochem J. 1988 Aug;20(8):427-32. doi: 10.1007/BF01002428.

DOI:10.1007/BF01002428
PMID:3058649
Abstract

This work describes a method for the immunolocalization of laminin on 1 micron-thick tissue sections using a postembedding immunofluorescence technique. Embedding of unfixed or formaldehyde-fixed mouse renal cortex in either of the acrylic resins LR-White or LR-Gold permitted reliable postembedding immunofluorescence staining for laminin. LR-White was heat-cured at 50 degrees C whereas LR-Gold was polymerized at -25 degrees C. A stronger immunostaining for laminin was obtained from tissue embedded in polymerized LR-Gold compared with the staining from tissue embedded in LR-White. Prerequisites for adequate postembedding immunostaining are the partial dehydration of the tissue (maximum ethanol concentration, 70%) and pepsin treatment of the tissue sections prior to performing the immunostaining reactions.

摘要

这项工作描述了一种使用包埋后免疫荧光技术在1微米厚的组织切片上进行层粘连蛋白免疫定位的方法。将未固定或甲醛固定的小鼠肾皮质包埋在丙烯酸树脂LR-White或LR-Gold中，均可对层粘连蛋白进行可靠的包埋后免疫荧光染色。LR-White在50℃加热固化，而LR-Gold在-25℃聚合。与LR-White包埋的组织染色相比，LR-Gold聚合包埋的组织对层粘连蛋白的免疫染色更强。进行充分的包埋后免疫染色的前提条件是组织的部分脱水（最大乙醇浓度为70%）以及在进行免疫染色反应之前对组织切片进行胃蛋白酶处理。

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1
LR-White and LR-Gold resins for postembedding immunofluorescence staining of laminin in mouse kidney.用于小鼠肾脏层粘连蛋白包埋后免疫荧光染色的LR-White和LR-金树脂。
Histochem J. 1988 Aug;20(8):427-32. doi: 10.1007/BF01002428.
2
Light and electron microscopical postembedding lectin histochemistry for WGA-binding sites in the renal cortex of the mouse embedded in polyhydroxy aromatic resins LR-White and LR-Gold.用多羟基芳香树脂LR-White和LR-Gold包埋的小鼠肾皮质中WGA结合位点的光镜和电镜后包埋凝集素组织化学研究
Histochemistry. 1988;89(3):277-82. doi: 10.1007/BF00493152.
3
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Methodological dependence in the ultrastructural immunolocalization of laminin in tubular basement membranes of the mouse kidney.小鼠肾脏肾小管基底膜中层粘连蛋白超微结构免疫定位的方法学依赖性
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Addition of phosphotungstic acid to ethanol for dehydration improves both the ultrastructure and antigenicity of pituitary tissue embedded in LR White acrylic resin.在用于脱水的乙醇中添加磷钨酸可改善包埋于LR White丙烯酸树脂中的垂体组织的超微结构和抗原性。
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Immunogold Staining of London Resin (LR) White Sections for Transmission Electron Microscopy (TEM).用于透射电子显微镜（TEM）的伦敦树脂（LR）白色切片的免疫金染色。
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引用本文的文献

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Appearance of laminin, heparan sulphate proteoglycan and collagen type IV during initial stages of vascularisation of the neuroepithelium of the mouse embryo.小鼠胚胎神经上皮血管形成初期层粘连蛋白、硫酸乙酰肝素蛋白聚糖和IV型胶原的出现情况。
J Anat. 1990 Apr;169:189-95.
3
Postembedding immunogold histochemistry for the localization of laminin and its E4 and P1 fragments in mouse kidney embedded in LR-White and LR-Gold.

本文引用的文献

1
Immunocytochemical enhancement of basement membrane antigens by pepsin: applications in diagnostic pathology.胃蛋白酶对基底膜抗原的免疫细胞化学增强作用：在诊断病理学中的应用
Am J Clin Pathol. 1984 Aug;82(2):191-4. doi: 10.1093/ajcp/82.2.191.
2
A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscope.一种用于在电子显微镜下快速显示组织抗原的简易包埋后系统。
Histochem J. 1983 Jun;15(6):543-55. doi: 10.1007/BF01954145.
3
Post-embedding immunolabelling. Some effects of tissue preparation on the antigenicity of plant proteins.
Histochem J. 1991 Jun;23(6):267-72. doi: 10.1007/BF01045045.
包埋后免疫标记。组织制备对植物蛋白抗原性的一些影响。
Eur J Cell Biol. 1982 Oct;28(2):251-6.
4
Demonstration of laminin, a basement membrane glycoprotein, in routinely processed formalin-fixed human tissues.在常规处理的福尔马林固定的人体组织中证实层粘连蛋白（一种基底膜糖蛋白）的存在。
Histochemistry. 1982;75(3):301-7. doi: 10.1007/BF00496733.
5
Immunocytochemical demonstration of serine: pyruvate amino-transferase in peroxisomes and mitochondria of rat kidney.大鼠肾脏过氧化物酶体和线粒体中丝氨酸：丙酮酸氨基转移酶的免疫细胞化学显示
Histochemistry. 1985;83(1):81-5. doi: 10.1007/BF00495305.
6
Methodological dependence in the ultrastructural immunolocalization of laminin in tubular basement membranes of the mouse kidney.小鼠肾脏肾小管基底膜中层粘连蛋白超微结构免疫定位的方法学依赖性
Histochemistry. 1987;87(1):59-64. doi: 10.1007/BF00518725.
7
Light and electron microscopical postembedding lectin histochemistry for WGA-binding sites in the renal cortex of the mouse embedded in polyhydroxy aromatic resins LR-White and LR-Gold.用多羟基芳香树脂LR-White和LR-Gold包埋的小鼠肾皮质中WGA结合位点的光镜和电镜后包埋凝集素组织化学研究
Histochemistry. 1988;89(3):277-82. doi: 10.1007/BF00493152.
8
Laminin--a glycoprotein from basement membranes.层粘连蛋白——一种来自基底膜的糖蛋白。
J Biol Chem. 1979 Oct 10;254(19):9933-7.