Migheli A, Attanasio A, Giordana M T
Neurological Clinic II, University of Torino, Italy.
Biotech Histochem. 1993 Mar;68(2):117-21. doi: 10.3109/10520299309104678.
A postembedding staining method is presented for ultrastructural visualization of amyloid deposits in brain sections from patients with Alzheimer's disease. Methenamine silver stain is applied to thin sections of tissue embedded in the acrylic resin LR Gold. Senile plaques are easily labeled by silver granules and the ultrastructural detail is well preserved. When staining time is prolonged, silver precipitate also is deposited on neuronal paired helical filaments. This method overcomes the drawbacks of previously reported applications of the stain on Vibratome and Epon sections. Thin sections from the same tissue block can be immunostained with antibodies to various plaque components, thus allowing comparative studies at the electron microscope level.
本文介绍了一种用于阿尔茨海默病患者脑切片中淀粉样沉积物超微结构可视化的包埋后染色方法。将六胺银染色应用于包埋在丙烯酸树脂LR Gold中的组织薄片。老年斑很容易被银颗粒标记，超微结构细节保存良好。当染色时间延长时，银沉淀也会沉积在神经元双螺旋丝上。该方法克服了先前报道的该染色法在振动切片机和环氧树脂切片上应用的缺点。同一组织块的薄片可以用针对各种斑块成分的抗体进行免疫染色，从而可以在电子显微镜水平上进行比较研究。
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