时间分辨荧光免疫法高效检测环境水样和土壤样品中的多效唑。
Highly efficient detection of paclobutrazol in environmental water and soil samples by time-resolved fluoroimmunoassay.
机构信息
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China; The Affiliated First People's Hospital of Jiangsu University, Zhenjiang 212002, China.
出版信息
Sci Total Environ. 2016 Nov 1;569-570:1629-1634. doi: 10.1016/j.scitotenv.2016.06.089. Epub 2016 Jun 16.
A fast and ultrasensitive indirect competitive time-resolved fluoroimmunoassay (TRFIA) was developed for the analysis of paclobutrazol in environmental water and soil samples. Paclobutrazol hapten was synthesized and conjugated to bovine serum albumin (BSA) for producing polyclonal antibodies. Under optimal conditions, the 50% inhibitory concentration (IC50 value) and limit of detection (LOD, IC20 value) were 1.09μgL(-1) and 0.067μgL(-1), respectively. The LOD of TRFIA was improved 30-fold compared to the already reported ELISA. There was almost no cross-reactivity of the antibody with the other structural analogues of triazole compounds, indicating that the antibody had high specificity. The average recoveries from spiked samples were in the range from 80.2% to 104.7% with a relative standard deviation of 1.0-9.5%. The TRFIA results for the real samples were in good agreement with that obtained by high-performance liquid chromatography analyses. The results indicate that the established TRFIA has potential application for screening paclobutrazol in environmental samples.
建立了一种用于环境水样和土壤样品中多效唑分析的快速、超灵敏的间接竞争时间分辨荧光免疫分析（TRFIA）方法。合成了多效唑半抗原，并与牛血清白蛋白（BSA）偶联，用于制备多克隆抗体。在最佳条件下，半数抑制浓度（IC50 值）和检测限（IC20 值，即 50%抑制浓度时的荧光强度）分别为 1.09μg/L 和 0.067μg/L。与已报道的 ELISA 相比，TRFIA 的检测限提高了 30 倍。抗体与三唑类化合物的其他结构类似物几乎没有交叉反应，表明该抗体具有高度特异性。加标样品的平均回收率在 80.2%至 104.7%之间，相对标准偏差为 1.0%至 9.5%。实际样品的 TRFIA 结果与高效液相色谱分析结果吻合良好。结果表明，建立的 TRFIA 方法具有在环境样品中筛选多效唑的潜力。
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