Zhang Zhen, Zhu Nuanfei, Huang Menglu, Liang Yaru, Zeng Kun, Wu Xiangyang, Liu Zhenjiang, Ma Qianchi, Qu Guangbo, Shi Jianbo
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P. O. Box 2871, Beijing 100085, China.
Environ Pollut. 2017 Oct;229:431-438. doi: 10.1016/j.envpol.2017.05.076. Epub 2017 Jun 14.
Tetrabromobisphenol A bis(2-hydroxyethyl) ether (TBBPA DHEE), as one of the TBBPA derivatives, is frequently applied as a flame retardant. To date, however, little evidence has been gathered regarding its environmental behavior and fate. This is mainly attributed to the lack of an analytical approach available to measure it. Mass spectrometry cannot determine levels of TBBPA DHEE. LC-MS could not ionize TBBPA DHEE to yield enough fragments, while GC-MS could not be applied for the analysis either, due to the thermal degradation of TBBPA DHEE. We here designed a novel hapten, then conjugated it with a carrier protein and produced a type of polyclonal antibody capable of recognizing TBBPA DHEE. Based on this approach, a sensitive and high-throughput indirect competitive enzyme-linked immunosorbent assay (ELISA) was established. The respective detection limit (LOD, based on 90% B/B) and IC calculated from the standard curve were 0.702 and 9.868 ng/mL for TBBPA DHEE. And the established method showed good tolerance to various matrix interferents. Using our ELISA, we measured the concentrations of these substances from environmental water samples with no pretreatment other than filtration. We found the presence of TBBPA DHEE in environmental water samples collected in Jiangsu province, and the detected concentrations ranging from 1.5 to 7.7 ng/mL. Our proposed method here could be applied to completely and systematically investigate TBBPA DHEE and TBBPA MHEE concentrations in environmental samples.
四溴双酚A双（2-羟乙基）醚（TBBPA DHEE）作为双酚A的衍生物之一，常被用作阻燃剂。然而，迄今为止，关于其环境行为和归宿的证据很少。这主要归因于缺乏可用于测量它的分析方法。质谱法无法测定TBBPA DHEE的含量。液相色谱-质谱联用（LC-MS）无法使TBBPA DHEE离子化以产生足够的碎片，而气相色谱-质谱联用（GC-MS）也无法用于分析，因为TBBPA DHEE会发生热降解。我们在此设计了一种新型半抗原，然后将其与载体蛋白偶联，制备了一种能够识别TBBPA DHEE的多克隆抗体。基于此方法，建立了一种灵敏且高通量的间接竞争酶联免疫吸附测定（ELISA）法。TBBPA DHEE的各自检测限（基于90% B/B）和从标准曲线计算出的IC分别为0.702和9.868 ng/mL。所建立的方法对各种基质干扰物具有良好的耐受性。使用我们的ELISA法，我们测量了环境水样中这些物质的浓度，除过滤外无需其他预处理。我们发现江苏省采集的环境水样中存在TBBPA DHEE，检测浓度范围为1.5至7.7 ng/mL。我们在此提出的方法可用于全面系统地研究环境样品中TBBPA DHEE和TBBPA MHEE的浓度。
