在发酵罐中通过均相培养生产分枝杆菌抗原。
The production of mycobacterial antigens by homogeneous culture in a fermentor.
作者信息
Nyabenda J, Bautens E, Borremans M, Verhofstadt R, Weckx M, Marchal A
机构信息
Pasteur Institute of Brabant, Tuberculin Laboratory, Brussels, Belgium.
出版信息
J Biol Stand. 1988 Oct;16(4):259-67. doi: 10.1016/0092-1157(88)90013-3.
Mycobacterium bovis strain BCG, substrain 1173P2, has been grown in homogeneous culture in classical synthetic Sauton medium without supplementary ingredients. The culture conditions are described. The protein release in the culture medium and the tuberculin yield after 2% trichloroacetic acid precipitation were significantly improved. The antigenicity of the tuberculin has been successfully assayed on specifically sensitized guinea-pigs. It is concluded that homogeneous mycobacterium culture in a fermentor using synthetic medium is a suitable method for the large scale production of antigen.
牛分枝杆菌卡介苗菌株1173P2在无补充成分的经典合成苏通培养基中进行了均匀培养。描述了培养条件。培养基中的蛋白质释放量以及经2%三氯乙酸沉淀后的结核菌素产量均有显著提高。已在特异性致敏豚鼠身上成功检测了结核菌素的抗原性。得出结论，在发酵罐中使用合成培养基进行分枝杆菌均匀培养是大规模生产抗原的合适方法。
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