对重组分枝杆菌抗原MPB64的迟发型超敏反应，在对结核分枝杆菌或牛分枝杆菌卡介苗致敏的豚鼠中。
Delayed-type hypersensitivity to a recombinant mycobacterial antigen, MPB64, in guinea pigs sensitized to Mycobacterium tuberculosis or Mycobacterium bovis BCG.
作者信息
Haga S, Yamaguchi R, Nagai S, Matsuo K, Yamazaki A, Nakamura R M
机构信息
Department of Bacteriology, National Institute of Health, Tokyo, Japan.
出版信息
J Leukoc Biol. 1995 Feb;57(2):221-5. doi: 10.1002/jlb.57.2.221.
Recombinant MPB64 (rMPB64), a mycobacterial antigen, was obtained from an Escherichia coli clone transformed with a recombinant expression vector, pMAL64c. The rMPB64 was examined for the activity to elicit delayed-type hypersensitivity (DTH) in guinea pigs injected with liver Mycobacterium tuberculosis H37Rv or live M. bovis BCG Tokyo. It was found that rMPB64 has the same reactivity as native MPB64 (nMPB64) or MPT64 (nMPT64) and the potency to elicit DTH was 13.4 times higher than that of PPD. Because MPB64 is secreted only by living M. tuberculosis and some strains of BCG, it is possible to use this antigen for the diagnosis of tuberculosis.
重组MPB64（rMPB64）是一种分枝杆菌抗原，它是从用重组表达载体pMAL64c转化的大肠杆菌克隆中获得的。对rMPB64进行了检测，以观察其在注射了结核分枝杆菌H37Rv肝脏菌液或活的牛型结核分枝杆菌东京株卡介苗的豚鼠中引发迟发型超敏反应（DTH）的活性。结果发现，rMPB64与天然MPB64（nMPB64）或MPT64（nMPT64）具有相同的反应性，且引发DTH的效力比结核菌素纯蛋白衍生物（PPD）高13.4倍。由于MPB64仅由活的结核分枝杆菌和某些卡介苗菌株分泌，因此有可能将这种抗原用于结核病的诊断。
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