70-kD热休克相关蛋白是至少两种不同的胞质因子之一，可刺激蛋白质导入线粒体。
70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria.
作者信息
Murakami H, Pain D, Blobel G
机构信息
Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York 10021.
出版信息
J Cell Biol. 1988 Dec;107(6 Pt 1):2051-7. doi: 10.1083/jcb.107.6.2051.
Abstract
We have developed an in vitro system in which the posttranslational import of Put2 (delta-pyrroline-5-carboxylate dehydrogenase), into yeast mitochondria is dependent on the addition of yeast postribosomal supernatant (PRS). When mRNA for a nuclear-encoded yeast mitochondrial matrix protein, Put2, was translated in a wheat germ cell-free system, import into posttranslationally added yeast mitochondria was negligible. However, when a yeast PRS was added, significant import was observed. The import stimulating activity of the yeast PRS was shown to consist of at least two distinct factors. One of these is the recently purified 70-kD heat shock-related protein Ssalp/Ssa2p, two proteins that are 98% homologous. The other factor is an N-ethylmaleimide-sensitive protein(s). Both factors act synergistically.
摘要
我们开发了一种体外系统，其中Put2（δ-吡咯啉-5-羧酸脱氢酶）向酵母线粒体的翻译后导入依赖于添加酵母核糖体后上清液（PRS）。当在麦胚无细胞系统中翻译核编码的酵母线粒体基质蛋白Put2的mRNA时，向翻译后添加的酵母线粒体中的导入可以忽略不计。然而，当添加酵母PRS时，观察到显著的导入。酵母PRS的导入刺激活性显示至少由两个不同的因子组成。其中之一是最近纯化的70-kD热休克相关蛋白Ssalp/Ssa2p，这两种蛋白有98%的同源性。另一个因子是对N-乙基马来酰亚胺敏感的蛋白。这两个因子协同作用。
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