风疹RNA和抗原在培养细胞中的原位检测。

In situ detection of rubella RNA and antigens in cultured cells.

作者信息

Filipenko D, Hobman T, MacDonald I, Gillam S

机构信息

Department of Pathology, Faculty of Medicine, University of British Columbia Research Centre, Vancouver, Canada.

出版信息

J Virol Methods. 1988 Oct;22(1):109-18. doi: 10.1016/0166-0934(88)90093-6.

DOI:10.1016/0166-0934(88)90093-6

PMID:3058736

Abstract

We developed in situ hybridization and immunofluorescent procedures to detect rubella RNA and antigens in tissue-cultured cells infected with rubella virus. cDNA fragments of the rubella virus E1 structural gene were used as probes for in situ hybridization to detect rubella RNA sequences in Vero cells infected with rubella virus. Using antibodies against rubella proteins, indirect immunofluorescence detected rubella virus structural proteins in Vero cells infected with rubella virus. The immunofluorescence method has also been applied to study the expression of rubella polypeptide E1 in transfected COS cells and may be applied to the detection and study of persistent rubella virus infection in human tissues.

摘要

我们开发了原位杂交和免疫荧光程序，以检测感染风疹病毒的组织培养细胞中的风疹RNA和抗原。风疹病毒E1结构基因的cDNA片段用作原位杂交的探针，以检测感染风疹病毒的Vero细胞中的风疹RNA序列。使用针对风疹蛋白的抗体，间接免疫荧光检测感染风疹病毒的Vero细胞中的风疹病毒结构蛋白。免疫荧光方法也已应用于研究转染的COS细胞中风疹多肽E1的表达，并可能应用于检测和研究人体组织中持续性风疹病毒感染。