Sifnaios Emmanouil, Mastorakos George, Psarra Katerina, Panagopoulos Nikolaos-Dimitrios, Panoulis Konstantinos, Vitoratos Nikolaos, Rizos Demetrios, Creatsas George
2nd Department of Obstetrics and Gynecology, Medical School, Aretaieion University Hospital, National and Kapodistrian University of Athens, Athens, Greece
Endocrine Unit, Medical School, Aretaieion University Hospital, National and Kapodistrian University of Athens, Athens, Greece.
In Vivo. 2019 Jan-Feb;33(1):31-40. doi: 10.21873/invivo.11435.
BACKGROUND/AIM: Gestational diabetes mellitus (GDM) is a common pregnancy complication, characterized by insulin resistance and low-grade systemic inflammation with a pro-inflammatory immune system response. Our objective was to study the peripheral Th1, Th2, Th17 and Treg response in GDM compared to normal pregnancy.
Th1, Th2, Th17 and Treg subsets was determined by flow cytometry based on staining for specific intracellular cytokines, as well as C-reactive protein (CRP) and total IgE circulating levels. The health status of all offspring was also assessed 6 months post-delivery.
A total of 49 Caucasian adult pregnant women were enrolled into a GDM (n=26) and Control (n=23) group. At the third trimester of pregnancy, the GDM group had a higher proportion of Th2, Th17 and Treg cells compared to control. Contrary to the control group, the GDM group exhibited no significant change in the Th1/Th2/Th17/Treg profile postpartum. Furthermore, higher circulating CRP and total IgE levels were noted in the GDM group compared to controls. At the 6-month post-delivery assessment, 30.8% of the offspring from the GDM group were found to have developed atopic dermatitis, food allergy or allergic proctocolitis compared to none from the control group.
Compared to an uncomplicated pregnancy, GDM exhibits a significantly different peripheral T-cell profile at the third pregnancy trimester characterized by higher proportion of Th2, Th17 and Treg cells which persist six months post-delivery, while the increased high sensitivity CRP (hsCRP) levels stressed the low-grade inflammatory profile of this disease.
背景/目的：妊娠期糖尿病（GDM）是一种常见的妊娠并发症，其特征为胰岛素抵抗以及伴有促炎免疫系统反应的低度全身炎症。我们的目的是研究与正常妊娠相比，GDM患者外周血中Th1、Th2、Th17和调节性T细胞（Treg）的反应。
通过基于特定细胞内细胞因子染色的流式细胞术，以及C反应蛋白（CRP）和总IgE循环水平，来确定Th1、Th2、Th17和Treg亚群。在分娩后6个月，还对所有后代的健康状况进行了评估。
共有49名白种成年孕妇被纳入GDM组（n = 26）和对照组（n = 23）。在妊娠晚期，与对照组相比，GDM组中Th2、Th17和Treg细胞的比例更高。与对照组相反，GDM组产后Th1/Th2/Th17/Treg细胞谱无显著变化。此外，与对照组相比，GDM组的循环CRP和总IgE水平更高。在产后6个月的评估中，发现GDM组30.8%的后代患有特应性皮炎、食物过敏或过敏性直肠结肠炎，而对照组无一例出现。
与未合并症的妊娠相比，GDM在妊娠晚期表现出显著不同的外周T细胞谱，其特征为Th2、Th17和Treg细胞比例更高，且这种情况在产后持续6个月，同时高敏CRP（hsCRP）水平升高凸显了该疾病的低度炎症特征。
