State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, Ministry of Agriculture/Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China.
College of Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Int J Mol Sci. 2018 Dec 26;20(1):93. doi: 10.3390/ijms20010093.
In plants, heptahelical proteins (HHPs) have been shown to respond to a variety of abiotic stresses, including cold stress. Up to the present, the regulation mechanism of HHP5 under low temperature stress remains unclear. In this study, was isolated from Pak-choi ( ssp. cv. ). Sequence analysis and phylogenetic analysis indicated that in Pak-choi is similar to in . Structure analysis showed that the structure of the BcHHP5 protein is relatively stable and highly conservative. Subcellular localization indicated that was localized on the cell membrane and nuclear membrane. Furthermore, real-time quantitative polymerase chain reaction (RT-qPCR) analysis showed that was induced to express by cold and other abiotic stresses. In Pak-choi, -silenced assay, inhibiting the action of endogenous , indicated that -silenced might have a negative effect on cold tolerance, which was further confirmed. All of these results indicate that might play a role in abiotic response. This work can serve as a reference for the functional analysis of other cold-related proteins from Pak-choi in the future.
在植物中，七螺旋跨膜蛋白（HHPs）已被证明对多种非生物胁迫（包括冷胁迫）有响应。截至目前，低温胁迫下 HHP5 的调控机制尚不清楚。本研究从白菜（ ssp. cv. ）中分离到 。序列分析和系统进化分析表明，白菜中的 与拟南芥中的 相似。结构分析表明，BcHHP5 蛋白的结构相对稳定且高度保守。亚细胞定位表明 位于细胞膜和核膜上。此外，实时定量聚合酶链反应（RT-qPCR）分析表明， 受冷胁迫和其他非生物胁迫诱导表达。在白菜中， -沉默试验抑制内源性 的作用，表明 -沉默可能对耐冷性有负面影响，这一点得到了进一步证实。所有这些结果表明， 可能在非生物响应中发挥作用。本研究可为今后白菜中其他与冷相关蛋白的功能分析提供参考。
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