Liu Chaoyang, Wang Xia, Xu Yuantao, Deng Xiuxin, Xu Qiang
Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, China,
Mol Biol Rep. 2014 Oct;41(10):6769-85. doi: 10.1007/s11033-014-3563-1. Epub 2014 Jul 10.
MYB transcription factor represents one of the largest gene families in plant genomes. Sweet orange (Citrus sinensis) is one of the most important fruit crops worldwide, and recently the genome has been sequenced. This provides an opportunity to investigate the organization and evolutionary characteristics of sweet orange MYB genes from whole genome view. In the present study, we identified 100 R2R3-MYB genes in the sweet orange genome. A comprehensive analysis of this gene family was performed, including the phylogeny, gene structure, chromosomal localization and expression pattern analyses. The 100 genes were divided into 29 subfamilies based on the sequence similarity and phylogeny, and the classification was also well supported by the highly conserved exon/intron structures and motif composition. The phylogenomic comparison of MYB gene family among sweet orange and related plant species, Arabidopsis, cacao and papaya suggested the existence of functional divergence during evolution. Expression profiling indicated that sweet orange R2R3-MYB genes exhibited distinct temporal and spatial expression patterns. Our analysis suggested that the sweet orange MYB genes may play important roles in different plant biological processes, some of which may be potentially involved in citrus fruit quality. These results will be useful for future functional analysis of the MYB gene family in sweet orange.
MYB转录因子是植物基因组中最大的基因家族之一。甜橙（Citrus sinensis）是全球最重要的水果作物之一，其基因组最近已被测序。这为从全基因组角度研究甜橙MYB基因的组织和进化特征提供了契机。在本研究中，我们在甜橙基因组中鉴定出100个R2R3-MYB基因。对该基因家族进行了全面分析，包括系统发育、基因结构、染色体定位和表达模式分析。基于序列相似性和系统发育，这100个基因被分为29个亚家族，高度保守的外显子/内含子结构和基序组成也有力支持了这一分类。甜橙与相关植物物种（拟南芥、可可和木瓜）之间MYB基因家族的系统基因组比较表明，进化过程中存在功能分化。表达谱分析表明，甜橙R2R3-MYB基因表现出明显的时空表达模式。我们的分析表明，甜橙MYB基因可能在不同的植物生物学过程中发挥重要作用，其中一些可能潜在地参与柑橘果实品质的形成。这些结果将有助于未来对甜橙MYB基因家族进行功能分析。
