Zhang Qi, Nie Jing, Chen Su-Juan, Li Qiang
Department of Natural Medicine, School of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350122, China,
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China,
Drug Des Devel Ther. 2018 Dec 19;13:71-77. doi: 10.2147/DDDT.S186029. eCollection 2019.
The aim of this study was to investigate the bioactive constituents of Qingwen Baidu Decoction (QBD) in a rat model of acute lung injury (ALI) induced by lipopolysaccharide (LPS). Our previous studies showed that ethyl gallate (EG) and pentagalloylglucose (PGG) were the active components of QBD for the treatment of ALI.
We isolated two compounds and identified the structures of them by nuclear magnetic resonance and mass spectrometer. Lung injury was induced by intratracheal instillation with LPS (5 mg/kg). Rats were randomly divided into six groups: Control group; LPS group; 5 mL/kg DEX + LPS group; 6.6 g/kg QBD extract + LPS group; 17.16 mg/kg PGG + LPS group; 7.26 mg/kg EG + LPS group. The effects of compounds on LPS-induced the number of total cells, neutrophils influx, protein leakage, W/D weight ratio and pulmonary histological changes were examined.
The results demonstrated that pretreatment with EG and PGG could notably inhibit lung edema and attenuate the pulmonary histological changes (<0.05). The pretreatment also decreased the number of total cells and polymorphonuclear leukocytes in bronchoalveolar lavage fluid (BALF) (<0.05).
Ethyl gallate and pentagalloylglucose of QBD played a protective role in preventing LPS-induced ALI. The results supported further study of EG and PGG as potential candidates for preventing ALI.
本研究旨在探讨清瘟败毒饮（QBD）在脂多糖（LPS）诱导的大鼠急性肺损伤（ALI）模型中的生物活性成分。我们之前的研究表明，没食子酸乙酯（EG）和五倍子酰葡萄糖（PGG）是QBD治疗ALI的活性成分。
我们分离出两种化合物，并通过核磁共振和质谱仪鉴定了它们的结构。通过气管内滴注LPS（5 mg/kg）诱导肺损伤。将大鼠随机分为六组：对照组；LPS组；5 mL/kg地塞米松+LPS组；6.6 g/kg QBD提取物+LPS组；17.16 mg/kg PGG+LPS组；7.26 mg/kg EG+LPS组。检测化合物对LPS诱导的总细胞数、中性粒细胞浸润、蛋白渗漏、湿干重比及肺组织学变化的影响。
结果表明，EG和PGG预处理可显著抑制肺水肿并减轻肺组织学变化（<0.05）。预处理还可减少支气管肺泡灌洗液（BALF）中总细胞和多形核白细胞的数量（<0.05）。
QBD中的没食子酸乙酯和五倍子酰葡萄糖在预防LPS诱导的ALI中发挥保护作用。结果支持进一步研究EG和PGG作为预防ALI的潜在候选药物。
