Taylor G M, Cook H T, Hanson C, Peart W S, Zondek T, Zondek L H
Medical Unit, St Mary's Hospital Medical School, Paddington, London, UK.
J Hypertens. 1988 Oct;6(10):845-51. doi: 10.1097/00004872-198810000-00012.
Human fetal lung homogenates contain an inactive form of renin which may be revealed by trypsin treatment. When activated, this form of renin has some biochemical similarities with fetal kidney renin: the pH optimum of fetal lung renin is approximately 6.5; it is bound by Affigel Blue affinity chromatography resin; and is inhibited by a monoclonal antibody (R-3-36-16) raised to human kidney renin. Inactive renin, partially-purified from both fetal kidney and lung, differs from this in that the renal form is of low-molecular weight (LMW, 45,000 daltons), whereas that from fetal lung is of high molecular weight (HMW, 58,000 daltons). Using a sensitive alkaline phosphatase-anti-alkaline phosphatase (APAAP) procedure with a polyclonal anti-renin antibody (R-15), immunoreactive renin in fetal lung was found in vessels in mesenchyme between airways. The pattern of staining was distributed similarly to Factor VIII-related antigen, suggesting localization in endothelial cells.
人胎儿肺匀浆含有一种无活性形式的肾素，胰蛋白酶处理可能会使其显现出来。这种形式的肾素被激活后，在一些生化特性上与胎儿肾肾素相似：胎儿肺肾素的最适pH约为6.5；它能与Affigel Blue亲和层析树脂结合；并被一种针对人肾肾素产生的单克隆抗体（R-3-36-16）所抑制。从胎儿肾脏和肺中部分纯化得到的无活性肾素与之不同，肾脏形式的肾素分子量较低（LMW，45,000道尔顿），而胎儿肺中的肾素分子量较高（HMW，58,000道尔顿）。使用一种敏感的碱性磷酸酶-抗碱性磷酸酶（APAAP）方法以及多克隆抗肾素抗体（R-15），发现胎儿肺中的免疫反应性肾素存在于气道间间质的血管中。染色模式与VIII因子相关抗原的分布相似，提示其定位于内皮细胞。
