Taylor G M, Cook H T, Sheffield E A, Hanson C, Peart W S
Medical Unit, St. Mary's Hospital Medical School, Paddington, London, England.
Am J Pathol. 1988 Mar;130(3):543-51.
The authors have used a sensitive alkaline phosphatase-anti-alkaline-phosphatase immunohistochemical method to examine 28 human pulmonary carcinomas for the presence of renin. Immunoreactive renin was found in 23 (82%) cases. Specific staining was always associated with small vessels in the stroma of the tumor or in adjacent areas of inflamed fibrous tissue. Within vessels, renin was localized in the cytoplasm of medial cells. Adenocarcinoma exhibited the most consistent staining (11/12 cases), and this appeared to be independent of the degree of tumor differentiation. Immunoreactive renin was also detected in squamous cell (7/8 cases), undifferentiated large cell (4/4 cases), and small cell undifferentiated carcinoma (1/1 cases), but the number of vessels and intensity of staining were usually less than seen in adenocarcinoma. Staining was not found in the bronchioloalveolar variant of adenocarcinoma (0/3 cases). By means of immunoaffinity chromatography with monoclonal antibodies (MAbs) raised to kidney renin, both active and inactive renin were extracted from homogenates of surgical specimens. The molecular weight of both forms of renin was approximately 59,000 daltons.
作者采用灵敏的碱性磷酸酶-抗碱性磷酸酶免疫组化方法，检测28例人类肺癌组织中肾素的存在情况。在23例（82%）病例中发现了免疫反应性肾素。特异性染色总是与肿瘤间质或邻近炎症性纤维组织区域的小血管相关。在血管内，肾素定位于中层细胞的细胞质中。腺癌显示出最一致的染色（11/12例），且这似乎与肿瘤分化程度无关。在鳞状细胞癌（7/8例）、未分化大细胞癌（4/4例）和小细胞未分化癌（1/1例）中也检测到免疫反应性肾素，但血管数量和染色强度通常低于腺癌。在细支气管肺泡腺癌变体中未发现染色（0/3例）。通过使用针对肾素产生的单克隆抗体（MAb）进行免疫亲和层析，从手术标本匀浆中提取了活性和非活性肾素。两种形式肾素的分子量均约为59,000道尔顿。
