Terech-Majewska Elżbieta, Siwicki Andrzej Krzysztof, Hermańska Sylwia, Kaczorek-Łukowska Edyta, Pajdak-Czaus Joanna, Schulz Patrycja
Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland.
Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland.
Cent Eur J Immunol. 2018;43(3):241-247. doi: 10.5114/ceji.2018.80041. Epub 2018 Oct 30.
The objective of this study was to investigate the effect of trans-resveratrol, a potent antioxidant with anti-inflammatory and chemopreventive properties, naturally occurring in many fruits and plants on lymphocytes proliferation and also on macrophages metabolic and phagocytic activity. The aim of this study was to demonstrate the immunomodulatory effects of the compound on fish immunocompetent cells and determine the type of this interaction (immunosuppression or immunostimulation). Proliferative activity of lymphocytes was studied by MTT assay, and the respiratory burst was evaluated using the respiratory burst activity (RBA) test. Phagocytic killing was tested using the PKA test. The experiment have shown that trans-resveratrol suppressed blood B cells, while there was no significant influence on blood T lymphocytes. However, insignificant stimulatory effect occurred at the lowest concentration. In addition, the compound inhibited proliferation of T and B lymphocytes isolated from the organs. Importantly, trans-resveratrol caused stimulation of blood and organs macrophages phagocytic killing, and also increased the respiratory burst of macrophages isolated from organ. These results suggest a potential use of trans-resveratrol as an immunomodulator of innate immunity in fish. This is particularly important, as this kind of resistance plays leading role in protecting the body against infection. In comparison, adaptive immunity is slower and also much less precise.
本研究的目的是调查反式白藜芦醇这种存在于许多水果和植物中的具有抗炎和化学预防特性的强效抗氧化剂，对淋巴细胞增殖以及巨噬细胞代谢和吞噬活性的影响。本研究的目的是证明该化合物对鱼类免疫活性细胞的免疫调节作用，并确定这种相互作用的类型（免疫抑制或免疫刺激）。通过MTT法研究淋巴细胞的增殖活性，并使用呼吸爆发活性（RBA）试验评估呼吸爆发。使用PKA试验测试吞噬杀伤作用。实验表明，反式白藜芦醇抑制血液中的B细胞，而对血液中的T淋巴细胞没有显著影响。然而，在最低浓度时出现了不显著的刺激作用。此外，该化合物抑制从器官分离的T和B淋巴细胞的增殖。重要的是，反式白藜芦醇刺激血液和器官巨噬细胞的吞噬杀伤作用，并且还增加从器官分离的巨噬细胞的呼吸爆发。这些结果表明反式白藜芦醇有可能作为鱼类先天免疫的免疫调节剂。这一点尤为重要，因为这种抵抗力在保护身体免受感染方面起主导作用。相比之下，适应性免疫反应较慢且准确性也低得多。
