氨酰-tRNA合成酶小片段的结晶

Crystallization of a small fragment of an aminoacyl tRNA synthetase.

作者信息

Frederick C A, Wang A H, Rich A, Regan L, Schimmel P

机构信息

Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

出版信息

J Mol Biol. 1988 Sep 20;203(2):521-2. doi: 10.1016/0022-2836(88)90019-8.

DOI:10.1016/0022-2836(88)90019-8

PMID:3058989

Abstract

Single crystals of an amino-terminal fragment of Escherichia coli alanine tRNA synthetase have been prepared by the vapor diffusion method. The fragment extends to amino acid residue 368 and catalyzes the synthesis of alanyl adenylate. The crystals grow in the presence of alanine as rhombic plates in space group P2(1)2(1)2(1) and with unit cell dimensions of a = 67.9 A, b = 98.5 A and c = 123.6 A (1 A = 0.1 nm). They diffract to better than 3 A resolution.

摘要

通过气相扩散法制备了大肠杆菌丙氨酸tRNA合成酶氨基末端片段的单晶。该片段延伸至氨基酸残基368，并催化丙氨酰腺苷酸的合成。晶体在丙氨酸存在下生长，呈菱形板状，空间群为P2(1)2(1)2(1)，晶胞参数为a = 67.9 Å，b = 98.5 Å，c = 123.6 Å（1 Å = 0.1 nm）。它们的衍射分辨率优于3 Å。