DU Ke, Zhou Yanheng, Chen Guoliang, He Xiaolong, Zhang Xiangqian
College of Life Science, Yan'an University, Yan'an 716000, China.
College of Life Science, Yan'an University, Yan'an 716000, China. *Corresponding author, E-mail:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2018 Nov;34(11):1021-1026.
Objective To investigate the effect of serpin E2 on the proliferation, invasion and migaration of colon cancer cells. Methods The expression of serpin E2 was detected by immunohistochemistry in 30 pairs of colon cancer and adjacent tissues. The expression of serpin E2 in colon cancer SW480 cells was down-regulated by siRNA. The down-regulation effect was verified by real-time fluorescent quantitative PCR and Western blot analysis. CCK-8 assay and plate cloning assay were used to detect the proliferation of colon cancer cells. Transwell assay was performed to detect the changes in cell invasion and migration. Results Serpin E2 was obviously overexpressed in the colon cancer tissue. The proliferation, invasion and migration ability of SW480 cells were inhibited significantly after the down-regulation of serpin E2 expression. Conclusion Knockdown of serpin E2 might inhibit the proliferation, invasion and migration of SW480 cells.
目的 探讨丝氨酸蛋白酶抑制剂E2（serpin E2）对结肠癌细胞增殖、侵袭及迁移的影响。方法 采用免疫组织化学法检测30对结肠癌组织及癌旁组织中serpin E2的表达。利用小干扰RNA（siRNA）下调结肠癌SW480细胞中serpin E2的表达，并通过实时荧光定量PCR及蛋白质免疫印迹法进行验证。采用CCK-8法及平板克隆法检测结肠癌细胞的增殖情况。进行Transwell实验检测细胞侵袭及迁移能力的变化。结果 serpin E2在结肠癌组织中明显高表达。下调serpin E2表达后，SW480细胞的增殖、侵袭及迁移能力均显著受到抑制。结论 敲低serpin E2可能抑制SW480细胞的增殖、侵袭及迁移。
