Hong Zhu, Zhang Xipeng
Department of Anal and Intestinal Surgery, Tianjin Union Medical Center (Nankai University Affiliated Hospital), Tianjin 300121, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2019 Jan 30;39(1):43-48. doi: 10.12122/j.issn.1673-4254.2019.01.07.
To investigate the expression of cytokine signal suppressor 3 (SOCS3) in colon cancer tissue and the mechanism by which SOCS3 regulates the proliferation and invasion of colon cancer.
We collected the specimens of tumor tissues and paired adjacent tissues from 80 patients with colon cancer undergoing radical resection in our hospital between July, 2014 and May, 2017, and the expression of SOCS3 in the tissue samples was analyzed using Western blotting. We also transfected colon cancer cell line SW480 with a SOCS3-overexpressing plasmid or a small interference RNA (siRNA) for SOCS3 knockdown, and the changes in the cell proliferation and invasion capacity were evaluated using CCK-8 assay and Transwell assay, respectively. The effect of demethylation and IL-6 treatment on SOCS3 expression and the proliferation and invasion of SW480 cells were observed.
Colon cancer tissues showed a lowered expression of SOCS3 compared with the adjacent tissues. Over-expression of SOCS3 significantly inhibited while SOCS3 knockdown obviously promoted the proliferation and invasion of SW480 cells . Demethylation treatment up-regulated SOCS3 expression and inhibited the proliferation and invasion capacity of SW480 cells; IL-6 treatment of the cells caused the reverse changes.
SOCS3 participates in the development and progression of colon cancer and serves as a potential target for colon cancer treatment. In patients with colon cancer, the low expression of SOCS3 possibly as a result of methylation may promote the proliferation and invasion of the cancer cells.
探讨细胞因子信号抑制因子3（SOCS3）在结肠癌组织中的表达情况以及SOCS3调控结肠癌增殖和侵袭的机制。
收集2014年7月至2017年5月在我院接受根治性切除术的80例结肠癌患者的肿瘤组织标本及配对的癌旁组织标本，采用蛋白质免疫印迹法分析组织样本中SOCS3的表达。我们还用SOCS3过表达质粒或用于敲低SOCS3的小干扰RNA（siRNA）转染结肠癌细胞系SW480，分别采用CCK-8法和Transwell法评估细胞增殖和侵袭能力的变化。观察去甲基化和IL-6处理对SW480细胞SOCS3表达及增殖和侵袭的影响。
与癌旁组织相比，结肠癌组织中SOCS3表达降低。SOCS3过表达显著抑制而敲低SOCS3明显促进SW480细胞的增殖和侵袭。去甲基化处理上调SOCS3表达并抑制SW'480细胞的增殖和侵袭能力；用IL-6处理细胞则导致相反的变化。
SOCS3参与结肠癌的发生发展，可作为结肠癌治疗的潜在靶点。在结肠癌患者中，SOCS3低表达可能由于甲基化导致，可促进癌细胞的增殖和侵袭。
