西红花酸治疗通过下调参与炎症的基因来抑制结肠癌细胞的增殖。
Crocetin treatment inhibits proliferation of colon cancer cells through down-regulation of genes involved in the inflammation.
作者信息
Zhuang Xinying, Dong Aihua, Wang Ruicai, Shi Aijian
机构信息
Department of Gastroenterology, People's Hospital of Linzi District, Zibo, Shandong Province 255400, China.
Department of Pathology, People's Hospital of LinziDistrict, Zibo, Shandong Province 255400, China.
出版信息
Saudi J Biol Sci. 2018 Dec;25(8):1767-1771. doi: 10.1016/j.sjbs.2017.04.005. Epub 2017 Apr 17.
BACKGROUND
The current study was designed to investigate the effect of crocetin on the proliferation inhibition of colon cancer cells and the underlying mechanism.
METHODS
MTT assay showed inhibition of proliferation of colon cancer cells in a dose based manner by crocetin treatment. At 30 µM concentration of crocetin proliferation rate of colon cancer cells was reduced to 14% after 24 h. Flow cytometry and fluorescence microscopy revealed induction of apoptosis in colon cancer cells on treatment with crocetin. The tube formation was suppressed significantly in the cultures of HUVEC treated with 30 µM concentration of crocetin compared to the control cultures.
RESULTS
The results from transwell assay revealed a significant reduction in the population of DU-145 cells passing through filters of transwell on treatment with crocetin compared to the control cells. Treatment of the DU-145 cells with crocetin caused a significant reduction in the expression levels of NF-κB, VEGF and MMP-9. The results from RT-PCR analysis revealed a significant reduction in the expression of genes involved in inflammation including, HMGB1, IL-6 and IL-8 on treatment of DU-145 cells with crocetin. However, the expression of NAG-1 gene was increased by crocetin treatment in DU-145 cells significantly compared to the control cells.
CONCLUSION
Crocetin inhibits growth of colon cancer cells and prevents tube formation through induction of apoptosis. Therefore, crocetin can be used efficiently for the treatment of colon cancer.
背景
本研究旨在探讨西红花酸对结肠癌细胞增殖抑制的作用及其潜在机制。
方法
MTT 法显示西红花酸处理可呈剂量依赖性抑制结肠癌细胞增殖。在 30 μM 浓度的西红花酸作用下，结肠癌细胞 24 小时后的增殖率降至 14%。流式细胞术和荧光显微镜检查显示，用西红花酸处理可诱导结肠癌细胞凋亡。与对照培养物相比，用 30 μM 浓度的西红花酸处理的人脐静脉内皮细胞（HUVEC）培养物中管腔形成明显受到抑制。
结果
Transwell 实验结果显示，与对照细胞相比，用西红花酸处理后，穿过 Transwell 滤膜的 DU-145 细胞数量显著减少。用西红花酸处理 DU-145 细胞导致 NF-κB、VEGF 和 MMP-9 的表达水平显著降低。RT-PCR 分析结果显示，用西红花酸处理 DU-145 细胞后，包括 HMGB1、IL-6 和 IL-8 在内的炎症相关基因的表达显著降低。然而，与对照细胞相比，西红花酸处理显著增加了 DU-145 细胞中 NAG-1 基因的表达。
结论
西红花酸通过诱导凋亡抑制结肠癌细胞生长并阻止管腔形成。因此，西红花酸可有效用于结肠癌的治疗。
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