Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran.
Asian Pac J Cancer Prev. 2024 Sep 1;25(9):3179-3185. doi: 10.31557/APJCP.2024.25.9.3179.
Crocin and Crocetin are compounds that have shown promising therapeutic potentials in various medical contexts. To date, the effect of crocin and crocetin on the expression level of miRNA-16-1 in Epstein Barr Virus (EBV)-induced lymphoma has not been investigated. This research delved into a comparative analysis of the cytotoxic effects of crocin and crocetin compared to cyclophosphamide, the main drug used in the treatment of lymphoma and PTLD, on B-cell lymphoma infected with EBV (cell line CO 88BV59-1). Additionally, the study examines the changes in miRNA-16-1 expression following these treatments in this cell line.
CO 88BV59-1 LCL cells were treated with crocin, crocetin (0.2 to 200 μM), and cyclophosphamide (0.05 to 50 μM) for 72 hours. Cell viability and apoptosis were assessed using resazurin and Annexin V/PI techniques, respectively. Additionally, the expression of miRNA-16-1-3p and miRNA-16-1-5p was determined using the Real-Time PCR method. The data were analyzed using one-way analysis of variance (ANOVA) with Tukey's multiple comparisons post-hoc test.
Crocin and crocetin inhibited the proliferation and apoptosis caused by EBV-infected cells in a dose- and time-dependent manner (P<0.05). The expression levels of miRNA-16-1-3p and miRNA-16-1-5p remained unchanged in cells treated with crocin and crocetin.
The study found that the cytotoxic effect of Crocin, Crocetin, and Cyclophosphamide on CO 88BV59-1 LCL is independent of the expression level of miRNA-16-1. The results showed a reduction in cell survival and an increase in cell death.
藏红花酸和藏红花素是在各种医学背景下显示出有前途的治疗潜力的化合物。迄今为止，藏红花酸和藏红花素对 Epstein Barr 病毒（EBV）诱导的淋巴瘤中 miRNA-16-1 表达水平的影响尚未得到研究。本研究深入探讨了藏红花酸和藏红花素与环磷酰胺（治疗淋巴瘤和 PTLD 的主要药物）相比，对 EBV 感染的 B 细胞淋巴瘤（细胞系 CO 88BV59-1）的细胞毒性作用的比较分析。此外，该研究还研究了在该细胞系中，在用这些药物处理后 miRNA-16-1 表达的变化。
用藏红花酸、藏红花素（0.2 至 200 μM）和环磷酰胺（0.05 至 50 μM）处理 CO 88BV59-1 LCL 细胞 72 小时。使用 Resazurin 和 Annexin V/PI 技术分别评估细胞活力和细胞凋亡。此外，使用实时 PCR 方法测定 miRNA-16-1-3p 和 miRNA-16-1-5p 的表达。使用单因素方差分析（ANOVA）和 Tukey 多重比较后检验对数据进行分析。
藏红花酸和藏红花素以剂量和时间依赖的方式抑制 EBV 感染细胞的增殖和凋亡（P<0.05）。藏红花酸和藏红花素处理的细胞中 miRNA-16-1-3p 和 miRNA-16-1-5p 的表达水平保持不变。
该研究发现 Crocin、Crocetin 和 Cyclophosphamide 对 CO 88BV59-1 LCL 的细胞毒性作用与 miRNA-16-1 的表达水平无关。结果表明细胞存活减少，细胞死亡增加。
