Haynes B F, Fauci A S
J Clin Invest. 1978 Mar;61(3):703-7. doi: 10.1172/JCI108982.
The present study was undertaken to determine the effect of in vivo hydrocortisone on the kinetics of subpopulations of normal human peripheral blood (PB) thymus-derived (T) cells. Normal volunteers received a single i.v. dose of hydrocortisone, and blood was taken just before, as well as 4, 24, and 48 h after hydrocortisone administration. T cells were purified from each specimen, and proportions and absolute numbers of T lymphocytes bearing receptors for the Fc portion of IgG (T(.G)) and for the Fc portion of IgM (T(.M)) were enumerated by rosetting T cells with bovine erythrocytes which had been coated with either antibovine erythrocyte IgG or IgM. 4 h after i.v. administration of hydrocortisone, T(.M) cells decreased from 52 (+/-5%) to 23 (+/-6%) of PB T cells (P < 0.01) and the absolute number of T(.M) cells decreased from 1,028 (+/-171) per mm(3) to 103 (+/-23) per mm(3) (P < 0.001). In contrast, relative proportion of T(.G) cells increased from 22 (+/-4%) to 66 (+/-7%), while the absolute numbers of T(.G) cells were essentially unchanged (P > 0.2). In vitro studies involving preincubation of T cells with hydrocortisone before rosette determination of T(.G) or T(.M) cells demonstrated that the decrease in absolute numbers of T(.M) cells did not represent hydrocortisone interference with T(.M) rosette formation, nor did it represent a switch of T(.M) cells to T(.G) cells. Thus, administration of hydrocortisone to normal subjects produces a selective depletion from the circulation of T lymphocytes which possess receptors for the Fc portion of IgM (T(.M) cells) and of T cells which possess no detectable F(C) receptor (T(.non-M, non-G) cells). T(.G) cells are relatively resistant to the lymphopenic effect of hydrocortisone. These data clearly demonstrate that in vivo corticosteroids have a differential effect on the kinetics of identifiable and distinct subsets of cells in the human T-cell class.
本研究旨在确定体内氢化可的松对正常人外周血（PB）胸腺来源（T）细胞亚群动力学的影响。正常志愿者静脉注射单次剂量的氢化可的松，在给药前以及给药后4小时、24小时和48小时采集血液。从每个样本中纯化T细胞，通过用包被有抗牛红细胞IgG或IgM的牛红细胞使T细胞形成玫瑰花结来计数携带IgG Fc段受体（T（.G））和IgM Fc段受体（T（.M））的T淋巴细胞的比例和绝对数量。静脉注射氢化可的松4小时后，T（.M）细胞从PB T细胞的52（±5%）降至23（±6%）（P<0.01），T（.M）细胞的绝对数量从每立方毫米1028（±171）降至每立方毫米103（±23）（P<0.001）。相反，T（.G）细胞的相对比例从22（±4%）增加到66（±7%），而T（.G）细胞的绝对数量基本不变（P>0.2）。在T（.G）或T（.M）细胞玫瑰花结测定前用氢化可的松对T细胞进行预孵育的体外研究表明，T（.M）细胞绝对数量的减少并不代表氢化可的松对T（.M）玫瑰花结形成的干扰，也不代表T（.M）细胞向T（.G）细胞的转变。因此，给正常受试者注射氢化可的松会导致循环中具有IgM Fc段受体的T淋巴细胞（T（.M）细胞）和没有可检测到的F（C）受体的T细胞（T（.非M，非G）细胞）选择性减少。T（.G）细胞对氢化可的松的淋巴细胞减少作用相对耐药。这些数据清楚地表明，体内皮质类固醇对人T细胞类中可识别和不同的细胞亚群动力学有不同的影响。
