School of Chemistry , David Brewster Road , University of Edinburgh , Edinburgh , EH9 3FJ , United Kingdom.
Institute for Integrated Micro and Nano Systems, School of Engineering , University of Edinburgh , Edinburgh , EH9 3JL , United Kingdom.
ACS Sens. 2019 Jan 25;4(1):185-191. doi: 10.1021/acssensors.8b01208. Epub 2019 Jan 11.
Morpholino Oligonucleotides (MOs), an uncharged DNA analogue, are functionalized with an acrylamide moiety and incorporated into polymer hydrogels as responsive cross-links for microRNA sequence detection. The MO cross-links can be selectively cleaved by a short target analyte single-stranded DNA (ssDNA) sequence based on microRNA, inducing a distinct swelling response measured optically. The MO cross-links offer significant improvement over DNA based systems through improved thermal stability, no salt requirement and 1000-fold improved sensitivity over a comparative biosensor, facilitating a wider range of sensing conditions. Analysis was also achieved using a mobile phone camera, demonstrating portability.
吗啉代寡核苷酸（MOs）是一种不带电荷的 DNA 类似物，其功能化带有丙烯酰胺部分，并掺入聚合物水凝胶中作为响应性交联剂，用于 microRNA 序列检测。MO 交联可以通过基于 microRNA 的短靶标分析物单链 DNA（ssDNA）序列选择性切割，通过光学测量诱导明显的溶胀响应。与 DNA 基系统相比，MO 交联通过提高热稳定性、无需盐要求以及相对于比较生物传感器提高 1000 倍的灵敏度提供了显著的改进，从而促进了更广泛的传感条件。还使用手机摄像头进行了分析，证明了便携性。
