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德国北海和波罗的海海洋哺乳动物物种中隐孢子虫属和贾第虫属的流行情况。

CRYPTOSPORIDIUM SPECIES AND GIARDIA SPECIES PREVALENCE IN MARINE MAMMAL SPECIES PRESENT IN THE GERMAN NORTH AND BALTIC SEAS.

作者信息

Grilo Miguel L, Gomes Lídia, Wohlsein Peter, de Carvalho Luís Madeira, Siebert Ursula, Lehnert Kristina

出版信息

J Zoo Wildl Med. 2018 Dec 13;49(4):1002-1006. doi: 10.1638/2017-0255.1.

Abstract

Marine mammals are known to harbor Cryptosporidium spp. and Giardia spp., especially species or genotypes commonly associated with humans. In animals of the North and Baltic Seas, this information is missing. In this study, fecal samples ( n = 97) from harbor porpoises ( Phocoena phocoena), harbor seals ( Phoca vitulina), and grey seals ( Phocoena phocoena) present in German waters were examined via microscopic examination (by using a modified Ziehl-Neelsen technique [MZN]) to estimate the prevalence of these parasites. Additionally, intestinal tissue samples ( n = 1,049) from specimens of 10 marine mammal species were submitted for histopathologic examination (HP). A low prevalence was detected (2.1 and 1.3%, for MZN and HP, respectively), associated with Cryptosporidium spp. Giardia spp. was not observed. Current results suggest a low prevalence of these parasites in marine mammals present in Germany. However, methods with higher sensitivity, such as molecular methods, are crucial to confirm the prevalence, establish origin routes, and determine epidemiologic factors associated with the low prevalence observed.

摘要

已知海洋哺乳动物携带隐孢子虫属和贾第虫属,尤其是那些通常与人类相关的物种或基因型。在北海和波罗的海的动物中,这方面的信息尚缺。在本研究中,通过显微镜检查(使用改良齐-尼氏技术[MZN])对德国海域的港湾鼠海豚(Phocoena phocoena)、港海豹(Phoca vitulina)和灰海豹(Phocoena phocoena)的粪便样本(n = 97)进行检查,以估计这些寄生虫的感染率。此外,还提交了10种海洋哺乳动物标本的肠道组织样本(n = 1,049)进行组织病理学检查(HP)。检测到的感染率较低(MZN和HP分别为2.1%和1.3%),与隐孢子虫属有关。未观察到贾第虫属。目前的结果表明,这些寄生虫在德国的海洋哺乳动物中感染率较低。然而,具有更高灵敏度的方法,如分子方法,对于确认感染率、确定起源途径以及确定与观察到的低感染率相关的流行病学因素至关重要。

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