Thema N, Tshilwane S I, Son L, Smith R M, Faber F E, Steyn H C, van Kleef M, Liebenberg J, Pretorius A
Agricultural Research Council-Onderstepoort Veterinary Research, Private Bag X5, Onderstepoort 0110, South Africa; Department of Veterinary Tropical Diseases, Veterinary Faculty, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa.
Agricultural Research Council-Onderstepoort Veterinary Research, Private Bag X5, Onderstepoort 0110, South Africa.
Vet Immunol Immunopathol. 2019 Jan;207:1-9. doi: 10.1016/j.vetimm.2018.11.012. Epub 2018 Nov 19.
Since CD8 T cells play an important role in resistance to infection with heartwater, effective vaccines against this disease will likely require identification of antigens that contain CD8 T cell epitopes responsible for cytotoxic T lymphocyte (CTL) responses. With the use of the fluorescent antigen-transfected target cell (FATT)-CTL assay, IFN-γ ELISPOT and flow cytometry, peptides that induce CTL, proliferation of CD8 + T cells and IFN-γ production were identified as possible target antigens for vaccine development. Of particular relevance was the finding that different peptides from different antigens were able to elicit varied cytotoxic activities by immune peripheral blood mononuclear cells (PBMC) from heartwater immune tick-infected sheep. Several peptides derived from Erum0660, Erum2330, Erum2540, Erum2580 and Erum5000 induced CTL in immune sheep PBMC. Peptide Erum2540-6 was the only peptide that induced significant CTL, CD8CD45RO and CD8IFN-γ by PBMC from all three sheep, and Erum2540 and p2540-20 induced the highest % CTL response in all three outbred sheep. These results suggest that these epitopes may be of major importance in heartwater recombinant vaccine development.
由于CD8 T细胞在抵抗心水病感染中发挥重要作用，有效的针对该病的疫苗可能需要鉴定出含有负责细胞毒性T淋巴细胞（CTL）反应的CD8 T细胞表位的抗原。通过使用荧光抗原转染靶细胞（FATT）-CTL检测、IFN-γ ELISPOT和流式细胞术，诱导CTL、CD8 + T细胞增殖和IFN-γ产生的肽被鉴定为疫苗开发的可能靶抗原。特别相关的是发现来自不同抗原的不同肽能够通过来自感染心水病的免疫蜱的绵羊的免疫外周血单核细胞（PBMC）引发不同的细胞毒性活性。源自Erum0660、Erum2330、Erum2540、Erum2580和Erum5000的几种肽在免疫绵羊PBMC中诱导CTL。肽Erum2540-6是唯一一种能在所有三只绵羊的PBMC中诱导显著CTL、CD8CD45RO和CD8IFN-γ的肽，并且Erum2540和p2540-20在所有三只远交绵羊中诱导的CTL反应百分比最高。这些结果表明这些表位在心水病重组疫苗开发中可能至关重要。
