Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Vet Microbiol. 2019 Jan;228:53-60. doi: 10.1016/j.vetmic.2018.11.013. Epub 2018 Nov 19.
Newcastle disease (ND) caused by infections with virulent strains of Newcastle disease virus (NDV) continues to be a threat for poultry industry worldwide. The prospect of developing a thermostable and effective NDV vaccine is still highly desirable. To investigate the determinants of thermostability in NDV, we generated recombinant NDV strains by exchanging viral hemagglutinin-neuraminidase (HN) gene or by mutating the fusion (F) gene. The results showed that the HN and F protein were both determinants of NDV thermostability. With increased thermostability, the HN protein-chimeric virus showed significantly reduced neuraminidase and hemadsorption activities, but its hemolytic activity was retained. We also found that changing the amino acid in the F protein cleavage sites, affected the thermostability as well as the pathogenicity and fusogenic capacity of the virus. Taken together, our results suggest that HN and F proteins both contribute to the thermostability of NDV, and other viral biological activities change as the thermostability of the virus changes. These findings should be of benefit to the development of a thermostable and efficacious NDV vaccine.
新城疫（ND）由强毒新城疫病毒（NDV）感染引起，仍然是全球家禽业的威胁。开发耐热且有效的 NDV 疫苗的前景仍然非常理想。为了研究 NDV 耐热性的决定因素，我们通过交换病毒血凝素-神经氨酸酶（HN）基因或突变融合（F）基因生成了重组 NDV 株。结果表明，HN 和 F 蛋白都是 NDV 耐热性的决定因素。随着耐热性的提高，HN 蛋白嵌合病毒的神经氨酸酶和血凝吸附活性显著降低，但溶血活性得以保留。我们还发现，改变 F 蛋白裂解位点的氨基酸会影响病毒的耐热性以及致病性和融合能力。总之，我们的研究结果表明，HN 和 F 蛋白均有助于 NDV 的耐热性，并且随着病毒耐热性的变化，其他病毒的生物学活性也会发生变化。这些发现应该有助于开发耐热且有效的 NDV 疫苗。
