Cobb Dustin A, Dandekar Aditya P, Hahn Young S
Beirne B. Carter Center for Immunology Research, University of Virginia, Charlottesville, VA, USA.
Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA.
Methods Mol Biol. 2019;1911:453-458. doi: 10.1007/978-1-4939-8976-8_31.
Hepatitis C virus-mediated immune suppression is an underlying feature leading to the establishment of viral persistence and chronic infection. In particular, HCV core protein has been shown to exhibit significant immunosuppressive activity of T cells and antigen presenting cells. Using an HCV core transgenic mouse system, in which liver hepatocytes express core protein, it is possible to study the effects of core-mediated immune suppression in vivo during viral infection. In this protocol, we describe the procedures for evaluating antigen-specific CD8 T cell responses in response to recombinant adenovirus infection in HCV core transgenic mice.
丙型肝炎病毒介导的免疫抑制是导致病毒持续存在和慢性感染的一个潜在特征。特别是，丙型肝炎病毒核心蛋白已被证明对T细胞和抗原呈递细胞具有显著的免疫抑制活性。利用一种丙型肝炎病毒核心转基因小鼠系统，其中肝脏肝细胞表达核心蛋白，就有可能在病毒感染期间体内研究核心介导的免疫抑制作用。在本方案中，我们描述了评估丙型肝炎病毒核心转基因小鼠对重组腺病毒感染的抗原特异性CD8 T细胞反应的程序。
