Department of Andrology, China-Japan Friendship Hospital, Beijing, 100029, China.
Department of Andrology, Chongqing Health Center for Women and Children, Chongqing, 400013, China.
Chin J Integr Med. 2019 Apr;25(4):292-297. doi: 10.1007/s11655-018-2915-9. Epub 2018 Dec 28.
To assess the effect of electroacupuncture (EA) on expression of cytoskeletal proteins from Sertoli cells (SCs) and spermatogenesis in rats with oligozoospermia of insufficiency of Shen (Kidney) essence syndrome (OIKES).
Twenty healthy male Sprague-Dawley rats were randomly assigned to four groups using a random number table: control, tripterygium glycosides (TG) treatment, sham and EA groups (n=5 in each group). A rat model of OIKES was established by oral gavage with TG. The EA group was treated with TG and received EA at Shenshu (BL 23) and Zusanli (ST 36) acupoints for 20 min, once daily for 30 days, while the sham group received EA at identical acupoints with skin penetration without stimulation. After 30 days, the final body weight and coefficients for the testis and epididymis were calculated and sperm parameters were measured. Immunohistochemical analyses were performed to detect expression of vimentin and α-tubulin in SCs and proliferating cell nuclear antigen (PCNA) immunoreactivity in germ cells. Apoptosis in germ cells was quantified by the transferase biotin-dUTP nick end labeling assay.
Compared with the control group, the final body weight and testis/epididymis coefficients of rats in the TG-treated group were not significantly different, but the sperm count and motility were lower (P<0.05). Expressions of vimentin and α-tubulin were also significantly weaker (P<0.01). The PCNA immunoreactivity of germ cells was decreased (P=0.059), whereas the apoptotic index of germ cells was increased significantly (P<0.01). In contrast, EA at BL 23 and ST 36 acupoints significantly improved the final body weight as well as the sperm count, concentration and motility (P<0.01 or P<0.05). EA increased expression of vimentin and α-tubulin in SCs markedly, and significantly enhanced PCNA immunoreactivity with decreased apoptosis in germ cells (P<0.01 or P<0.05).
EA at BL 23 and ST 36 acupoints has protective effects on spermatogenesis in rats with OIKES. This effect seems to be achieved by attenuating TG-induced disruption of cytoskeletal protein in SCs.
评估电针对少精子症肾（肾）精不足证（OIKES）大鼠睾丸支持细胞（SCs）骨架蛋白表达和生精的影响。
采用随机数字表法将 20 只健康雄性 Sprague-Dawley 大鼠随机分为 4 组：对照组、雷公藤多苷（TG）治疗组、假手术组和电针组（每组 5 只）。通过灌胃 TG 建立 OIKES 大鼠模型。电针组在 TG 治疗的同时给予电针肾俞（BL 23）和足三里（ST 36）穴，每次 20 分钟，每日 1 次，共 30 天，假手术组给予相同穴位皮肤穿透但无刺激的电针。30 天后，计算终体重和睾丸附睾系数，测量精子参数。免疫组化法检测 SC 中波形蛋白和α-微管蛋白的表达以及生精细胞中增殖细胞核抗原（PCNA）的免疫反应性。通过末端转移酶生物素-dUTP 缺口末端标记法检测生精细胞的凋亡。
与对照组相比，TG 治疗组大鼠的终体重和睾丸附睾系数无显著差异，但精子计数和活力较低（P<0.05）。波形蛋白和α-微管蛋白的表达也明显减弱（P<0.01）。生精细胞的 PCNA 免疫反应性降低（P=0.059），而生精细胞的凋亡指数显著增加（P<0.01）。相比之下，BL 23 和 ST 36 穴位电针显著提高了终体重以及精子计数、浓度和活力（P<0.01 或 P<0.05）。电针显著增强了 SC 中波形蛋白和α-微管蛋白的表达，明显增强了生精细胞的 PCNA 免疫反应性，降低了生精细胞的凋亡（P<0.01 或 P<0.05）。
BL 23 和 ST 36 穴位电针对 OIKES 大鼠生精有保护作用。这种作用似乎是通过减轻 TG 诱导的 SC 中细胞骨架蛋白的破坏来实现的。
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