Xu Ya-ping, Liu Bao-xing, Zhang Xiu-ping, Yang Chao-wei, Wang Chuan-hang
Institute of Clinical Medicine, China-Japan Friendship Hospital, Beijing, 100029, China.
Chin J Integr Med. 2014 Mar;20(3):194-9. doi: 10.1007/s11655-014-1743-4. Epub 2014 Mar 4.
To evaluate the effects of the Chinese herbal formula Wuzi Yanzong Pill (, WYP) on the spermatogenesis and specific secretory functions of Sertoli cells in rat model and to investigate the underlying mechanism.
Five groups of male Sprague-Dawley rats including the control group, the model group, the low-dose WYP group, the medium-dose WYP group and the high-dose WYP group (5 in each group) were treated daily with vehicle, multiglycosides of Tripterygium wilfordii Hook f (GTW) either alone (20 mg/kg) or followed by WYP (0.5, 1.0, or 2.0 g/kg daily), respectively for 30 days. Serum levels of follicle-stimulating hormone (FSH), inhibin B (INHB) and testosterone (T) were evaluated using enzyme-linked immunosorbent assay. Androgen-binding protein (ABP) gene expression and transferrin (TF) protein expression in testis tissue specimens of all rats were determined using real-time reverse transcriptase polymerase chain reaction and Western blotting analysis, respectively. Histopathological alterations in the testis were determined using Johnsen's score.
The toxicity of GTW towards Sertoli cell secretory functions and spermatogenesis was accompanied by increased serum FSH concentrations and decreased INHB and T concentrations. Upregulated ABP mRNA levels, and decreased TF protein expression and Johnsen's scores were detected in the model group compared with the control group P<0.05 or P<0.01). Oral high-dose WYP administrations to GTW-treated rats effectively alleviated all of the GTW-induced changes in specific secretory functions of Sertoli cells (ABP, INHB and TF). Furthermore, serum T level and Johnsen's score of the testis increased greatly compared with the model group (P<0.01).
WYP has the ability to improve the spermatogenesis, possibly through modulating the secretory proteins expression of Sertoli cells.
评估中药方剂五子衍宗丸（WYP）对大鼠模型生精作用及支持细胞特定分泌功能的影响，并探讨其潜在机制。
将雄性Sprague-Dawley大鼠分为五组，即对照组、模型组、低剂量WYP组、中剂量WYP组和高剂量WYP组（每组5只），分别每日给予溶媒、雷公藤多苷（GTW）单独处理（20 mg/kg）或随后给予WYP（分别为0.5、1.0或2.0 g/kg/天），持续30天。采用酶联免疫吸附测定法评估血清卵泡刺激素（FSH）、抑制素B（INHB）和睾酮（T）水平。分别采用实时逆转录聚合酶链反应和蛋白质印迹分析测定所有大鼠睾丸组织标本中雄激素结合蛋白（ABP）基因表达和转铁蛋白（TF）蛋白表达。采用约翰森评分法确定睾丸的组织病理学改变。
GTW对支持细胞分泌功能和生精作用的毒性伴随着血清FSH浓度升高以及INHB和T浓度降低。与对照组相比，模型组检测到ABP mRNA水平上调，TF蛋白表达降低，约翰森评分降低（P<0.05或P<0.01）。对接受GTW处理的大鼠口服高剂量WYP可有效缓解GTW诱导的支持细胞特定分泌功能（ABP、INHB和TF）的所有变化。此外，与模型组相比，血清T水平和睾丸约翰森评分显著升高（P<0.01）。
WYP具有改善生精作用的能力，可能是通过调节支持细胞的分泌蛋白表达来实现的。
