Gestational exposure to acrylamide inhibits mouse placental development in vivo.

Acrylamide, a carcinogen and neurotoxic substance, recently has been discovered in various heat-treated carbohydrate-rich foods. The aim of this study was to investigate the effects of acrylamide exposure on placental development. Pregnant mice received acrylamide by gavage at dosages of 0, 10, and 50 mg/kg/day from gestational days (GD) 3 until GD 8 or GD 13. The results showed that acrylamide feeding significantly decreased the numbers of viable embryos and increased the numbers of resorbed embryos on GD 13. Acrylamide exposure reduced the absolute and relative weight of placentas and embryos, and inhibited the development of ectoplacental cone (EPC) and placenta, as shown by the atrophy of EPC and reduced placental area. Acrylamide markedly reduced the numbers of labyrinth vessels. Expression levels of most placental key genes such as Esx1, Hand1, and Hand2 mRNA dramatically decreased in acrylamide-treated placentas. Furthermore, acrylamide treatment inhibited proliferation and induced apoptosis of placentas, as shown by decreased Ki67-positive cells and Bcl-2 protein, and increased the expression of Bax, cleaved-caspase-3, and cleaved-caspase-8 proteins. In conclusion, our results indicated that gestational exposure to acrylamide inhibits placental development through dysregulation of placental key gene expression and labyrinth vessels, suppression of proliferation, and apoptosis induction in mice.