Department of Thoracic Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, China (mainland).
Med Sci Monit. 2018 Dec 31;24:9504-9508. doi: 10.12659/MSM.896748.
BACKGROUND The aim of this study was to detect the expression of fork-head box D3 (FOXD3) and investigate its diagnostic value in patients with non-small cell lung cancer (NSCLC). MATERIAL AND METHODS The relative expression of FOXD3 at mRNA and protein levels was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blotting analysis, respectively. Chi-square test was used to explore the relevance of FOXD3 expression with clinical features of NSCLC patients. A receiver operating characteristic (ROC) curve was built to estimate the diagnostic value of FOXD3 in distinguishing NSCLC patients from healthy controls. RESULTS Serum FOXD3 expression was weakly expressed in NSCLC patients compared to the controls at mRNA and protein levels (P<0.001) and low FOXD3 expression was positively correlated with TNM stage, lymph node metastasis, and differentiation. The ROC curve indicated that FOXD3 acts as a diagnostic bio-marker for NSCLC patients, with an AUC of 0.826 corresponding to a sensitivity of 77.1% and a specificity of 74.6%, and an optimal cutoff point of 2.38. CONCLUSIONS Decreased expression of serum FOXD3 was observed in NSCLC patients, and it was found to be a potential molecular marker for the diagnosis of NSCLC.
本研究旨在检测叉头框蛋白 D3（FOXD3）的表达，并探讨其在非小细胞肺癌（NSCLC）患者中的诊断价值。
采用实时定量逆转录聚合酶链反应（qRT-PCR）和 Western blot 分析分别检测 FOXD3 在 mRNA 和蛋白水平的相对表达。采用卡方检验探讨 FOXD3 表达与 NSCLC 患者临床特征的相关性。构建受试者工作特征（ROC）曲线评估 FOXD3 区分 NSCLC 患者与健康对照者的诊断价值。
与对照组相比，NSCLC 患者血清 FOXD3 的表达在 mRNA 和蛋白水平均较弱（P<0.001），并且低 FOXD3 表达与 TNM 分期、淋巴结转移和分化呈正相关。ROC 曲线表明 FOXD3 可作为 NSCLC 患者的诊断生物标志物，AUC 为 0.826，灵敏度为 77.1%，特异性为 74.6%，最佳截断点为 2.38。
NSCLC 患者血清 FOXD3 表达降低，可能是 NSCLC 诊断的潜在分子标志物。
