State Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming 650091, PR China.
FEMS Microbiol Lett. 2019 Jan 1;366(2). doi: 10.1093/femsle/fny287.
The rhizosphere bacterium ZKB-2 showed strong nematostatic activity against Meloidogyne incognita. Our study aimed to identify the nematostatic metabolites and evaluate the biocontrol efficiency in pot experiments. As the bacterial culture filtrate showed 100% nematostatic activity against M. incognita juveniles in 12 hr, we isolated and identified six compounds following activity guiding. 3-methoxycyclobutane-1, 2-dione showed 58.9% and 72.2% nematostatic activities against juveniles of M. incognita in 12 and 48 hr, with strong LC50 value at 447 μg mL-1. In pot experiments, treatments with the bacterial culture filtrate of strain ZKB-2 showed significant efficacy, especially at doses of 150 mL/pot, which were close to that of avermectin (positive control) at 0.01 g kg-1 soil. The most effective treatment inhibited 85.1% population of juveniles of M. incognita in the roots and 76.9% in the rhizosphere soil after 30 days. Furthermore, the promoting tomato growth also significantly increased in a dose-dependent manner. Our results revealed the potential of strain ZKB-2 to act as a biocontrol agent in the integrated management of root-knot nematodes on tomatoes.
根际细菌 ZKB-2 对南方根结线虫具有强烈的杀线虫活性。本研究旨在鉴定杀线虫代谢产物，并通过盆栽试验评估其生防效率。由于细菌培养液滤液在 12 小时内对南方根结线虫幼虫表现出 100%的杀线虫活性，我们在活性指导下分离并鉴定了六种化合物。3-甲氧基环丁烷-1,2-二酮对南方根结线虫幼虫在 12 和 48 小时时的杀线虫活性分别为 58.9%和 72.2%，LC50 值在 447μg mL-1 时较强。在盆栽试验中，ZKB-2 菌株的细菌培养液处理表现出显著的防治效果，特别是在 150 mL/盆的剂量下，与 0.01 g kg-1 土壤的阿维菌素（阳性对照）非常接近。最有效的处理在 30 天后，在根和根际土壤中抑制了南方根结线虫幼虫 85.1%的种群。此外，番茄生长的促进也呈剂量依赖性显著增加。我们的结果表明，ZKB-2 菌株有望成为番茄根结线虫综合管理中的一种生防剂。
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