Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois.
Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IIllinois.
Biotechnol Bioeng. 2019 Apr;116(4):904-911. doi: 10.1002/bit.26907. Epub 2019 Feb 4.
L-Fucose (6-deoxy-L-galactose) is a major constituent of glycans and glycolipids in mammals. Fucosylation of glycans can confer unique functional properties and may be an economical way to manufacture L-fucose. Research can extract L-fucose directly from brown algae, or by enzymatic hydrolysis of L-fucose-rich microbial exopolysaccharides. However, these L-fucose production methods are not economical or scalable for various applications. We engineered an Escherichia coli strain to produce L-fucose. Specifically, we modified the strain genome to eliminate endogenous L-fucose and lactose metabolism, produce 2'-fucosyllactose (2'-FL), and to liberate L-fucose from 2'-FL. This E. coli strain produced 16.7 g/L of L-fucose with productivity of 0.1 g·L ·h in a fed-batch fermentation. This study presents an efficient one-pot biosynthesis strategy to produce a monomeric form of L-fucose by microbial fermentation, making large-scale industrial production of L-fucose feasible.
L-岩藻糖（6-脱氧-L-半乳糖）是哺乳动物糖链和糖脂的主要成分。糖链的岩藻糖化可以赋予独特的功能特性，并且可能是制造 L-岩藻糖的经济方法。研究可以直接从褐藻中提取 L-岩藻糖，或通过酶解富含 L-岩藻糖的微生物胞外多糖。然而，这些 L-岩藻糖生产方法在各种应用中并不经济或可扩展。我们设计了一种大肠杆菌菌株来生产 L-岩藻糖。具体来说，我们修饰了菌株基因组以消除内源性 L-岩藻糖和乳糖代谢，生产 2'-岩藻糖（2'-FL），并从 2'-FL 中释放 L-岩藻糖。该大肠杆菌菌株在分批补料发酵中生产了 16.7 g/L 的 L-岩藻糖，生产率为 0.1 g·L ·h。本研究提出了一种通过微生物发酵生产 L-岩藻糖单体的高效一锅合成策略，使大规模工业生产 L-岩藻糖成为可能。
