State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, PR China.
J Appl Microbiol. 2019 Apr;126(4):1199-1208. doi: 10.1111/jam.14190. Epub 2019 Jan 24.
Spodoptera exigua is a serious pest of many agricultural crops. However, the bacterial communities of S. exigua are poorly studied, particularly over their entire life cycle. We aimed to study the biodiversity of the microbiota across the life cycle of S. exigua and to provide a better and obtain insight into new pest control strategies.
The bacterial diversity across the life cycle of S. exigua was studied using Illumina MiSeq sequencing of 16S rRNA genes. Spodoptera exigua is dominated by Proteobacteria and Firmicutes, with a total relative abundance of 90·03%. Enterococcus (24·6%), Pseudomonas (12·2%) and Asaia (45·9%) were abundant and active in eggs, while Methylobacterium (18·7%) and Halomonas (16·5%) dominated freshly eclosed larvae. The 3rd and 5th instar larvae were dominated by Enterococcus (76·3 and 62·0%). Pupal stages had the highest microbial diversity. There was no significant difference between newly emerged males and females. Symbionts of eggs were extremely similar and probably vertically transmitted by males during mating.
The result showed that the bacterial community was affected by the host developmental stages. Our results also suggest that symbionts of egg mass are probably vertically transmitted control by male spawning adults.
Our study documents the symbiont bacteria across the life cycle of S. exigua. Understanding the microbial symbionts may provide clues to develop potential biocontrol techniques against this pest.
甜菜夜蛾是许多农业作物的严重害虫。然而，甜菜夜蛾的细菌群落研究甚少，特别是在其整个生命周期中。我们旨在研究甜菜夜蛾整个生命周期中微生物群落的生物多样性，并提供更好的了解，以获得新的害虫控制策略。
使用 Illumina MiSeq 测序 16S rRNA 基因研究了甜菜夜蛾整个生命周期中的细菌多样性。甜菜夜蛾主要由变形菌门和厚壁菌门组成，相对丰度总计为 90.03%。肠球菌（24.6%）、假单胞菌（12.2%）和亚洲菌（45.9%）在卵中丰富且活跃，而甲基杆菌（18.7%）和盐单胞菌（16.5%）在刚孵化的幼虫中占优势。第 3 龄和第 5 龄幼虫以肠球菌（76.3%和 62.0%）为主。蛹期的微生物多样性最高。新羽化的雌雄个体之间没有显著差异。卵的共生体非常相似，可能通过雄虫交配时垂直传播。
结果表明，细菌群落受宿主发育阶段的影响。我们的研究结果还表明，卵群的共生体可能通过雄性产卵成虫垂直传播进行控制。
本研究记录了甜菜夜蛾整个生命周期中的共生细菌。了解微生物共生体可能为开发针对这种害虫的潜在生物防治技术提供线索。
