Zhang Bingchen, Yang Weicheng, He Qinqin, Chen Hangdan, Che Bingjie, Bai Xiaojie
School of Life Sciences, Guizhou Normal University, Guiyang, Guizhou, China.
Guizhou Chishui Alsophila National Nature Reserve Administration Bureau, Chishui, Guizhou, China.
Front Microbiol. 2024 Jun 19;15:1392586. doi: 10.3389/fmicb.2024.1392586. eCollection 2024.
As an indispensable part of insects, intestinal symbiotic bacteria play a vital role in the growth and development of insects and their adaptability. , the main pest of the relict plant , poses a serious threat to the development of the population. In the present study, 16S rDNA and internal transcribed spacer high-throughput sequencing techniques were used to analyze the structure of intestinal microbes and the diversity of the insect feeding on two different plants, as well as the similarities between the intestinal microorganisms of . The dominant bacteria of leaf endophytes were also compared based on the sequencing data. The results showed that Proteobacteria, Firmicutes, and Actinobacteria were the dominant phyla of intestinal bacteria, and Ascomycota was the dominant phylum of intestinal fungi. , , and were the dominant genera in the intestine of . feeding on two plants, and the relative abundance was significantly different between the two groups. was the common dominant genus of intestinal fungi in the two groups, and no significant difference was observed in its abundance between the two groups. This showed that compared with the intestinal fungi of . , the abundance of the intestinal bacteria was greatly affected by food. The common core microbiota between the microorganisms in leaves and the insect gut indicated the presence of a microbial exchange between the two. The network correlation diagram showed that the gut microbes of . feeding on were more closely related to each other, which could help the host to better cope with the adverse external environment. This study provides a theoretical basis for the adaptation mechanism of . and a new direction for the effective prevention and control of . .
作为昆虫不可或缺的一部分，肠道共生细菌在昆虫的生长发育及其适应性方面发挥着至关重要的作用。，这种残遗植物的主要害虫，对种群的发展构成了严重威胁。在本研究中，利用16S rDNA和内部转录间隔区高通量测序技术分析了以两种不同植物为食的昆虫肠道微生物的结构、多样性以及肠道微生物之间的相似性。还根据测序数据比较了叶片内生菌的优势细菌。结果表明，变形菌门、厚壁菌门和放线菌门是肠道细菌的优势门类，子囊菌门是肠道真菌的优势门类。，，和是肠道中的优势属。以两种植物为食，两组之间的相对丰度存在显著差异。是两组肠道真菌的共同优势属，两组之间其丰度未观察到显著差异。这表明与的肠道真菌相比，肠道细菌的丰度受食物影响较大。叶片中的微生物与昆虫肠道之间的共同核心微生物群表明两者之间存在微生物交换。网络相关图显示，以植物为食的昆虫肠道微生物之间的相关性更强，这有助于宿主更好地应对不利的外部环境。本研究为的适应机制提供了理论依据，并为的有效防治提供了新方向。
