CIMAR/CIIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, Portugal; Instituto de Ciências Biomédicas Abel Salazar Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
CIMAR/CIIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, Portugal.
Ecotoxicol Environ Saf. 2019 Mar;169:894-901. doi: 10.1016/j.ecoenv.2018.11.078. Epub 2018 Nov 29.
This study aimed to evaluate the response of bacteria, in terms of microbial community structure, from constructed wetland (CW) microcosms exposed to two veterinary antibiotics, enrofloxacin (ENR) and ceftiofur (CEF), alone or in a mixture, identifying which bacterial groups were dominant in CWs substrate during livestock wastewater treatment. Wastewater, not-doped or doped with ENR and/or CEF (100 µg/L each), was treated during 18 one-week cycles. Systems showed removal percentages > 85% for the added antibiotics, showing also high removal percentages for nutrients and organic matter and confirming CWs systems were working properly. However, both time of exposure and presence of antibiotics influenced significantly CWs substrate bacterial community structure. Pyrosequencing results showed bacterial communities were dominated by phyla Proteobacteria (38-48%), Firmicutes (20-27%), Bacteroidetes (12-15%) and Actinobacteria (4-9%), and that their relative abundance was clearly affected by the presence of the antibiotics. Results suggest the applicability of CWs for the removal of veterinary antibiotics from livestock wastewaters and provide new knowledge about the bacteria within the system, which can potentially be involved in removal processes. This information could in the future be used to improve CWs removal rates of pharmaceuticals from livestock wastewaters.
本研究旨在评估细菌的反应，就微生物群落结构而言，从人工湿地（CW）微宇宙中暴露于两种兽医抗生素，恩诺沙星（ENR）和头孢噻呋（CEF），单独或混合，确定在处理家畜废水中哪种细菌群落在 CWs 基质中占优势。在 18 个为期一周的周期中，用未掺杂或掺杂 ENR 和/或 CEF（各 100μg/L）的废水进行处理。系统对添加的抗生素的去除率>85%，对营养物质和有机物的去除率也很高，证实 CWs 系统运行正常。然而，暴露时间和抗生素的存在都显著影响了 CWs 基质细菌群落结构。焦磷酸测序结果表明，细菌群落主要由变形菌门（38-48%）、厚壁菌门（20-27%）、拟杆菌门（12-15%）和放线菌门（4-9%）组成，其相对丰度明显受到抗生素的存在影响。结果表明 CWs 可用于从家畜废水中去除兽医抗生素，并提供有关系统中细菌的新知识，这些细菌可能参与去除过程。这些信息将来可用于提高 CWs 从家畜废水中去除药物的效率。
