Babatabar Saeme, Zamir Seyed Morteza, Shojaosadati Seyed Abbas, Yakhchali Bagher, Zarch Ali Babaei
Biotechnology Group, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
Biotechnology Group, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
J Biosci Bioeng. 2019 Jun;127(6):732-737. doi: 10.1016/j.jbiosc.2018.12.001. Epub 2018 Dec 29.
Bisphenol A (BPA) is a toxic compound emitting to the environment mainly by polycarbonate production facilities. In this research, BPA with the initial concentrations in the range of 1-40 mg l was degraded by Ralstonia eutropha. The bacteria were unable to use BPA as the sole carbon source. Therefore, resting and growing cells of phenol-adapted R. eutropha were used for cometabolic biodegradation of BPA with phenol at the concentration of 100 mg l. The optimum initial concentrations of BPA were 20 mg l in both approaches of cometabolism. By using resting cells, BPA removal efficiency (RE) reached to 57%, however, RE decreased to 37% by growing cells in the presence of phenol. BPA-degrading activity was inhibited at BPA concentrations >20 mg l. Liquid chromatography-mass spectrometry technique was used to identify some metabolic intermediates generated during BPA degradation process as 1,2-bis(4-hydroxyphenyl)-2-propanol, 4-(2-propanol)-phenol, 4-hydroxyacetophenone, 4-isopropenylphenol, and 4-hydroxybenzoic acid. Finally, metabolic pathways for BPA degradation were proposed in this study.
双酚A（BPA）是一种主要由聚碳酸酯生产设施排放到环境中的有毒化合物。在本研究中，初始浓度在1-40毫克/升范围内的双酚A被真养产碱杆菌降解。该细菌无法将双酚A作为唯一碳源。因此，用适应苯酚的真养产碱杆菌的静止细胞和生长细胞对浓度为100毫克/升的苯酚进行双酚A的共代谢生物降解。在两种共代谢方法中，双酚A的最佳初始浓度均为20毫克/升。使用静止细胞时，双酚A的去除效率（RE）达到57%，然而，在苯酚存在下生长细胞时，RE降至37%。当双酚A浓度>20毫克/升时，双酚A降解活性受到抑制。采用液相色谱-质谱技术鉴定了双酚A降解过程中产生的一些代谢中间体，如1,2-双（4-羟苯基）-2-丙醇、4-（2-丙醇）-苯酚、4-羟基苯乙酮、4-异丙烯基苯酚和4-羟基苯甲酸。最后，本研究提出了双酚A的降解代谢途径。
