College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541006, People's Republic of China; The Guangxi Key Laboratory of Theory and Technology for Environmental Pollution Control, Guilin, 541006, People's Republic of China.
College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541006, People's Republic of China; Guangxi Key Laboratory of Electrochemical and Magnetochemical Functional Materials, Guilin, 541006, People's Republic of China.
Chemosphere. 2019 Jun;224:743-750. doi: 10.1016/j.chemosphere.2019.02.143. Epub 2019 Feb 23.
As a typical class of environmental endocrine disruptors, bisphenol A poses a potential threat to the sustainable survival and reproduction of living beings and human beings. In this study, the interaction between Trametes versicolor laccase and bisphenol A (BPA) was studied by molecular docking simulation, and the catalytic degradation of BPA was verified by experiments. The conditions for the laccase production of T. versicolor were optimized by orthogonal design, and the degradation of BPA was studied using its crude enzyme solution. The optimum degradation conditions were obtained by response surface methodology (RSM). Ultimately, the transformation products after 3 and 6 h of reaction were detected by gas chromatography-mass spectrometry. Docking results demonstrated that the reaction between laccase and BPA was spontaneous, and the degradation rate in 24 h reached 88.76%. RSM results showed that the highest BPA degradation rate of 97.68% was reached after 1 h reaction at 44.6 °C, 5 mg/L initial BPA concentration, and pH 5.20. The intermediate products of BPA catalyzed by laccase included ethylbenzene, p-xylene, and cyclohexanone 1-methyl-4-isopropenyl-2-cyclohexenol. This finding reveals that BPA degradation by the crude laccase from T. versicolor starts from the C atoms between two benzene rings that connect BPA. Compared with expensive pure enzyme, the crude laccase solution prepared by T. versicolor showed greater efficiency in BPA degradation. This work provides theoretical references and experimental methods for the biological processing of harmful pollutants.
双酚 A 作为一类典型的环境内分泌干扰物，对生物及人类的可持续生存和繁衍存在潜在威胁。本研究通过分子对接模拟研究了杂色云芝漆酶与双酚 A（BPA）的相互作用，并通过实验验证了 BPA 的催化降解。采用正交设计优化了杂色云芝产漆酶的条件，并利用其粗酶液研究了 BPA 的降解。采用响应面法（RSM）获得了最佳降解条件。最终，通过气相色谱-质谱法检测了反应 3 和 6 h 后的转化产物。对接结果表明，漆酶与 BPA 的反应是自发的，24 h 内的降解率达到 88.76%。RSM 结果表明，在 44.6°C、初始 BPA 浓度为 5 mg/L 和 pH 值为 5.20 的条件下，反应 1 h 后 BPA 的最高降解率达到 97.68%。漆酶催化 BPA 的中间产物包括乙苯、对二甲苯和环己酮 1-甲基-4-异丙烯基-2-环己烯醇。这一发现表明，杂色云芝粗漆酶对 BPA 的降解从连接 BPA 的两个苯环之间的 C 原子开始。与昂贵的纯酶相比，杂色云芝制备的粗漆酶溶液在 BPA 降解方面表现出更高的效率。这项工作为有害污染物的生物处理提供了理论参考和实验方法。
